Superior Detectability. Enhanced Quality. Consistent Results.
Miniaturization at the 10–15 µl level maintains the statistical analysis with sufficient beads present
Excellent retention of beads, even after extensive washing
In side-by-side comparisons, DropArray™ plates match standard microplates both in levels of sensitivity and quality of results. Furthermore, they show consistency assay after assay.
It is well known that Luminex assays suffer from bead aggregation with plasma, blood, and other challenging biological samples which affects detectability of proteins and jeopardizes quality of data. DroArray eliminates bead aggregation and improves the quality of data offering increased detectability and consistency of results.
The Curiox DA-Bead Plate vs. Traditional Plate Technology
(A) Incubation method principle in traditional plate (left) and DA-Bead Plate (right). Beads are freely floating with shaking in traditional well at 75µl volume vs. beads are fixed in a 15 µl drop in DA-Bead Plate by a magnet below the well and shaken.
(B) Washing method principle in traditional plate (left) and DA-Bead Plate (right). Magnet array with traditional plate either super packs beads at the side of a traditional well or keep beads as a monolayer resulting in aspiration of beads and/or incomplete washing with the nozzle/pipet based dispenser. Conversely, DA-Bead Plate magnet configuration maintains beads as a monolayer (right) resulting in complete washing with gentle laminar flow and minimal residual wash buffer leftover.
More Data Points Using Less Sample and Reagents. Significant Savings.
A 5-fold miniaturization reduces total assay costs by 60–80%
DropArray™ plates allow 5x more wells to be run from a single assay kit
Multiplex Luminex® assays are robust and generate highly sensitive data from a variety of samples types. However, these assays are costly and involve expensive reagents and often valuable samples. Curiox’s new DropArray™ plates allow users to analyze the same number of analytes at a fraction of the cost and sample consumption. DropArray™ plates use 5-fold smaller volumes, so the same amount of reagent can produce 5x more data points.
Easy Set Up. Flexible Format. Compatible Design.
DropArray™ plates are fully compatible with existing workflows
Use your established, standard protocols with DropArray™ plates. The overall workflow remains the same. Available in 96-well format, the plates are offered individually or in bulk to suit your assay needs. Because they are easy to use with standard robotics, therefore there’s no need for additional capital investment. DropArray™ plates are compatible with both polystyrene and magnetic beads. In addition, DropArray™ plates can be quickly washed with a convenient washer that avoids unnecessary bead loss, providing additional cost savings.
5x savings on magnetic multiplexing beads and sample volume. A conventional microtiter plate (MTP) running 25 µl sample + 25 µl beads, compared to Curiox DA-Bead plates running 5 µl sample + 5 µl beads (top). When plotted against one another, DA-Bead plates and MTP plates show practically identical correlation (bottom graphs).
How DA-Bead Plates Stack Up
|DA-Bead Plate (96-well plate)||MTP (96-well plate)|
|Sample volume||10 µl*||50 µl*|
|Bead volume||5 µl*||25 µl*|
|Sensitivity||Same as MTP or improved||N/A|
|Cross-reactivity||Same as MTP or improved||N/A|
|Ease of washing||More convenient, faster||N/A|
|No. of wells per kit||480||96|
|Total reagent cost of one 96-well assay||$300||$1500|
|Wash of non-magnetic beads||Easy||Difficult|
|*These values represent volumes from eBioscience’s Procartaplex™ kit.|