Webinar On-Demand: Shift the Paradigm to Centrifuge-less Immuno-staining of Cells for Superior Viability, Recovery & Data

CELL AND GENE THERAPY
Get fast, consistent and gentle cell processing for highly reproducible workflows

Technology

Gentle washing for critical workflows in cell-based therapeutics.

Benefits

Lower the CVs and save FTE time from research to manufacturing.

Scientific Data

Real world data from cell therapy companies.

Resources

Links to related content for cell and gene therapy.

Overview

CHANGE THE WAY YOU PROCESS CELLS TO GET RELIABLY CONSISTENT DATA

Clear resolution and retention of rare cell populations proves to be an issue in cell therapy processing using traditional centrifugation protocols, which can cause significant mechanical stress, reduced viability, and low retention of target cells. This is particularly problematic when dealing with rare cell types like gamma-delta T cells, which tend to have low starting cell numbers and high variability. It is therefore critical to be able to resolve these populations clearly to ensure the optimal cell therapy is delivered to the right patient.

New and improved with a touchscreen for easy menu selection, as well as a buffer exchanger for automated startup and shutdown sequences, the HT2000 washer is available as a stand-alone platform or as part of a fully-automated solution – the AUTO1000™.

The Laminar Wash™ HT2000 washer provides a proven alternative to the comparatively inconsistent centrifugal process and its associated washing steps. It ensures reproducible, automated washes across operators and similar or improved cell retention and viability. By eliminating the need for plate flicking, it greatly reduces the risks of cross-contamination and biosafety hazard to the scientist. Its trademark benefits of efficient debris removal while preserving cell state makes for cleaner separation of rare cell populations. These advantages have been documented by an increasing number of laboratories that have adopted the Laminar Wash™ technology in their workflow.

“The Laminar Wash’s performance allows us to get very tight control over populations with very tight CV’s largely due to the fact of the clear separations between populations. The maintained ability to detect rare populations is important as we detect the next round of cell therapy products.”
Arnaud Colantonio, PhD. Associate Director of Analytical Development at AdiCet Bio, now with Kite Pharma

TRADITIONAL

CENTRIFUGATION

VS

LAMINAR FLOW

TECHNOLOGY

Comparison of an immuno-staining workflow for the preparation of a single cell suspension for flow cytometry analysis. Laminar Wash technology is equally effective in washing but without the use of a centrifuge.

Benefits
BENEFITS OF LAMINAR WASH FOR CELL AND GENE THERAPY

The use of Laminar Wash can improve data from research to analytical development to manufacturing quality control. The Laminar Wash technology can provide the following to cell and gene therapy applications:

  • Lower CVs
  • Improved reproducibility among operators, times, and locations
  • Reduce hands-on time
  • Save FTE time
Featured Resource
CENTRIFUGE-LESS RED BLOOD CELL LYSIS AND IMMUNOSTAINING WHOLE BLOOD FOR FLOW CYTOMETRY USING LAMINAR WASH™ TECHNOLOGY
Scientific Data
DATA USING THE LAMINAR WASH SYSTEMS
Improved Resolution of Rare Cell Populations
Laminar Wash™ consistently gives better resolution between populations and less spread within populations, making gating strategy easier for operators and algorithms. Data shown here of rare gamma-delta T cell types, courtesy of Adicet Bio, a Curiox customer.
Improved Condition of Rare Cells

Laminar Wash™ enables better preservation of rare cell types. Data shown here of MAIT, Treg, and gamma-delta T cells in a whole blood depletion assay.

FEATURED RESOURCES
ALL YOU NEED TO KNOW ABOUT THE LAMINAR WASH™ HT2100 SYSTEM
Walk-Away Automation of Cell Immunostaining Assays by Centrifuge-Less Laminar Wash™
Centrifuge-less Whole Blood Staining with Laminar Wash in a Biosafety Cabinet
Intergrating Centrifuge Independent and Time Efficient Automated Washing in Flow Cytometry Crossmatching
RELATED PRODUCTS AND ACCESSORIES
LAMINAR WASH HT2000
A reliable and easy-to-use benchtop instrument for high throughput flow cytometry labs.
LAMINAR WASH AUTO 1000
Complete Automation Solution for Surface Staining and Intracellular Staining for Flow Cytometry.
LAMINAR MINI SYSTEM
An affordable, benchtop instrument for low-throughput sample preparation for flow cytometry.
Get Started
AUTOMATE YOUR FLOW CYTOMETRY WORKFLOW