AUTOMATED WORKFLOWS
Fully automate your workflows with an alternative (at last!) to centrifugation.
Laminar Wash technology makes automation easy compared to integrating a centrifuge.
Automate your workflow and save FTE time.
Data from applications that benefit from automation.
Links to useful content regarding automation.
Overview
CHANGE THE WAY YOU PROCESS CELLS TO GET RELIABLY CONSISTENT DATA.
AUTOMATED SAMPLE PREP
The Laminar Wash™ technology consists of a wall-less plate design and a laminar-flow cell washer that enables washing of suspension cells at a fraction of the hands-on time while consistently producing higher cell retention and viability. Using a total of 192 individual nozzles, the Laminar Wash™ washer uses a gentle flow of buffer across the top of settled cells to wash away debris and unbound antibodies while avoiding the significant stress placed on cells during traditional centrifugation methods. The result is reliable, consistent, and superior data in flow cytometry, single-cell sequencing, or other downstream applications.
The Laminar Wash HT2000 can be easily integrated into virtually any robotic arm or personal pipettor workstation. The HT2000 Automation License Key includes an API available for developers to integrate the HT2000 system. A fast and simple automated workflow can wash 10 plates in just over 20 minutes total time. And, many of the challenges faced when integrating centrifuges are eliminated since there are no rotor positions or aspirations to perform.
“I think the Laminar Wash technology is a prime device for creating an automated flow cytometry station ”
Dr. Arnuad Colantonio, AdiCet Bio, now with Kite Pharma
Technology
LAMINAR WASH TECHNOLOGY INTEGRATION WITH ROBOTICS
Centrifuges have been the only solution to automate suspension cell assays due to the need to wash cells during many assays. However, centrifuges have a number of limitations and require many steps to integrate. Centrifugation of suspension cells is a roadblock to otherwise easy automation.
Laminar Wash technology systems can be easily integrated into automation systems for methods such as flow cytometry, single cell sequencing, mass cytometry and in application areas such as CMC/Process Development, COVID-19/infectious diseases, tumor microenvironment, vaccine development.
Benefits
BENEFITS OF LAMINAR WASH FOR AUTOMATED WORKFLOWS
Laminar Wash technology makes it easy for applications that require a high degree of automation such as screening final product for cell therapeutics. Laminar Wash system integration can replace centrifuge integrations and only require one robotic movement instead of over 20 movements. Integration using Laminar Wash technology can:
- Reduce hands-on time and save FTE resources
- Lower the CVs of results
- Easily allow integration on-deck or off-deck
Featured Resource
S-LAB PRO WITH A CURIOX LAMINAR WASH HT2000 INTEGRATION
Scientific Data
DATA USING THE LAMINAR WASH SYSTEMS
Reproducibility of Low Numbers of Cells
Laminar Wash™ shows consistently higher cell retention compared to traditional centrifugation, even at low cell numbers. Data shown using isolated naive T-cells from freshly-thawed PBMCs, at starting cell numbers of 10k, 50k, and 100k. Actual cell counts of CD3+ and %CD3+ in singlets demonstrate consistent higher cell retention rates compared to equal numbers of washes.
Increased Reproducibility of Data from Critical Samples
Laminar Wash™ technology demonstrates greater reproducibility between replicates compared to traditional centrifugation. Data shown here in a flow cytometric crossmatch assay (FXCM), widely used in human leukocyte antigen (HLA) labs to predict donor-recipient compatibility. Using three test sera and two controls in eight replicates, LW™ produced a lower coefficient of variance in B-cell median channel fluorescence (MCF) values of 7.9%, versus 12.4% in the centrifugation method. Higher MCF values on the positive control also gave FCXM greater sensitivity, which is of great value in clinical settings.
FEATURED RESOURCES
ALL YOU NEED TO KNOW ABOUT THE LAMINAR WASH HT2000 SYSTEM
Recent Publications View All Publications >
Recent Publications View All Publications >
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AUTOMATE YOUR FLOW CYTOMETRY WORKFLOW
