Fully automate cell preparation workflows with an upgrade from centrifugation.
Laminar Wash technology makes automation easy compared to integrating a centrifuge.
Automate your workflow and save FTE time.
Data from applications that benefit from automation.
Links to useful content regarding automation.
CHANGE THE WAY YOU PROCESS CELLS TO GET RELIABLY CONSISTENT DATA.
AUTOMATED SAMPLE PREP
“I think the Laminar Wash technology is a prime device for creating an automated flow cytometry station ”
Dr. Arnuad Colantonio, formally of AdiCet Bio,
now with Kite Pharma
LAMINAR WASH TECHNOLOGY INTEGRATION WITH ROBOTICS
Besides the undue stress that centrifuges impart on cells during washing steps, they also require several complicated steps that are difficult to automate. Centrifugation of suspension cells is a roadblock to otherwise straightforward automation.
Laminar Wash systems are easily automated to support flow cytometry, single cell sequencing, mass cytometry, as well as applications including CMC/Process Development, COVID-19/infectious diseases, tumor microenvironment, and vaccine development.
BENEFITS OF LAMINAR WASH FOR AUTOMATED WORKFLOWS
Laminar Wash technology makes it easy for applications that require a high degree of automation such as screening final product for cell therapeutics. Laminar Wash systems can replace centrifuge integrations and only require one robotic movement instead of over 20 movements. Integration using Laminar Wash technology can:
- Reduce hands-on time and save FTE resources
- Lower the CVs of results
- Easily accomodate on-deck or off-deck automation
Building a custom assay for flow cytometry with the Laminar Wash AUTO1000 User Interface
DATA USING THE LAMINAR WASH SYSTEMS
Reproducibility of Low Numbers of Cells
Laminar Wash consistently yields higher cell retention compared to the traditional centrifuge, even at low cell numbers. Presented are isolated naive T-cells from freshly-thawed PBMCs, at starting cell numbers of 10k, 50k, and 100k. Actual cell counts of CD3+ and %CD3+ in singlets demonstrate consistently higher cell retention rates compared to equal numbers of washes.
Increased Reproducibility of Data from Critical Samples
Laminar Wash technology demonstrates greater reproducibility between replicates compared to traditional centrifugation. Presented here is a flow cytometric crossmatch assay (FXCM), widely used in human leukocyte antigen (HLA) labs to predict donor-recipient compatibility. Using three test sera and two controls in eight replicates, Laminar Wash produced a lower coefficient of variance in B-cell median channel fluorescence (MCF) values of 7.9%, versus 12.4% in the centrifugation method. Higher MCF values on the positive control also gave greater FCXM sensitivity, which is highly valued in clinical settings.
ALL YOU NEED TO KNOW ABOUT THE LAMINAR WASH HT2000 SYSTEM
AUTOMATE YOUR FLOW CYTOMETRY WORKFLOW