COVID-19 & INFECTIOUS DISEASE

Improve biosafety by reducing the generation of aerosols from infectious samples.

COVID-19 & Infectious Disease-resize

Process infectious samples in a biosafety hood.

Increase safety of sample preparation and reduce debris.

See examples of cleaner samples.

Links to useful resources for infectious sample processing.

Overview

CHANGE THE WAY YOU PROCESS CELLS TO
GET RELIABLY CONSISTENT DATA.

The World Health Organization Laboratory guidance for research on the novel coronavirus SARS-CoV2 is that “ all technical procedures should be performed in a way that minimizes the generation of aerosols and droplets”

Table 1. Risk prioritization of selected routine laboratory tasks
Task or activity Exposure risk
Potential hazard Likelihood Consequence Risk rating
Subculturing blood culture bottle Needle stick — percutaneous inoculation Likely Infection; medical treatment High
Aerosols — inhalation Moderate Infection; medical treatment Medium
Splash — direct contact with mucous membranes Moderate Infection; medical treatment High
Centrifugation Aerosols — inhalation Likely Infection; medical treatment High
Performing Gram stain Aerosols from flaming slides Moderate Colonization; infection Moderate
Preparing AFB smear only Aerosols from sputum or slide preparation Likely Illness; medical treatment; disease High
Performing catalase testing Aerosols — mucous membrane exposure Unlikely Colonization; infection Low
AFB culture work-up Aerosols — inhalation Likely Illness; medical treatment; disease High
Abbreviation: AFB = acid-fast bacillus.

Curiox Biosystems introduces a new method to prepare immune profiling cell populations within COVID-19 or other infectious disease samples by containing infectious disease blood sample processing within a biosafety cabinet from start to finish, all without the use of a centrifuge, thus eliminating aerosolization of potentially infectious samples.

This new application for infectious disease sample handling combines a new configuration of the Laminar Wash HT2000 system packaged with STEMCELL Technologies EasySep RBC depletion reagent resulting in increased viability and cell retention by reducing stress on already fragile cells that can come from harsh methods such as red blood cell lysis with associated repeated centrifugation steps. This natural and gentle cell prep method using Laminar Wash technology results in superior data and shows improved staining resolution of rare and sensitive immune cellular subsets, like monocytes and neutrophils, that are known to play a crucial role in the immune response against COVID-19 infections.

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“The Laminar Washer is safer than flicking (minimal exposure to infectious materials) and requires less space than centrifuges.”

Blanca Ponce-Ngo, Montefiore Medical Center
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Technology

TRADITIONAL CENTRIFUGATION

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LAMINAR WASH TECHNOLOGY

Comparison of an immuno-staining workflow for the preparation of a single cell suspension for flow cytometry analysis. Laminar Wash technology is equally effective in washing but without the use of a centrifuge.

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Benefits

BENEFITS OF LAMINAR WASH FOR SINGLE CELL SEQUENCING & PROTEOGENOMICS

The compact size of the Laminar Wash HT2000 or Laminar Wash MINI allow it to easily fit into a biosafety hood. Laminar Wash technology can improve infectious disease sample preparation by:

  • Enhancing the safety of the workflow by containing in a hood
  • Reduce aerosol production from samples
  • Reduce debris, background and clogging of instruments
  • Improve resolution and stain index
Featured Resource

CENTRIFUGE-LESS RED BLOOD CELL LYSIS AND IMMUNOSTAINING WHOLE BLOOD FOR FLOW CYTOMETRY USING LAMINAR WASH™ TECHNOLOGY

CELL LYSIS
Scientific Data

DATA USING THE LAMINAR WASH SYSTEMS

LW is Gentle to Neutrophils and Monocytes

Using 100μL of human whole blood, a comparison study using a 16-color immune profiling panel was conducted to compare LaminarWash to centrifugation protocols. Using Laminar Wash technology, there is increased retention of both the monocyte and neutrophil populations using Laminar Wash utilizing one-step washing, thus minimizing cell perturbations when compared to multiple centrifugation steps.

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Better Debris Removal and Cell Retention

Data adapted from the Curiox Biosystems webinar by Dr. Christoph S. Eberle from the Discovery Research Services at Charles River Laboratories, Worcester, MA When performing cell dissociation in tumor bearing tissues, the tissue debris and enzymatic-induced fragments are primary factors affecting cell recovery for tumor infiltrating lymphocytes. For more invasive and immunologically challenging models, like B16F10-inoculated mice, recovery of limited numbers of TILs reduces chances for accurate characterization. The results shown here were demonstrated with an unchallenged mouse sample and the Laminar Wash technology. Laminar washing (with 1,000,000 tumor infiltrate cells) reveals increased viable cell count and reduced debris effects by laminar washing. The cleaner sample preparation increases lymphocyte recovery up to 90.6% as compared to centrifuge-harsh washing of 10.5%.
Data adapted from the Curiox Biosystems webinar by Dr. Christoph S. Eberle from the Discovery Research Services at Charles River Laboratories, Worcester, MA

Recent Publications View All Publications >

    Peritoneal Fluid Cytokines Reveal New Insights of Endometriosis Subphenotypes

    Trispecific antibodies enhance the therapeutic efficacy of tumor-directed T cells through T cell receptor co-stimulation

    2020 | Nature Cancer

    Post-hoc assessment of the immunogenicity of three antibodies reveals distinct immune stimulatory mechanisms

    2019 | JoVE

    TLR4 agonist protects against Trypanosoma cruzi acute lethal infection by decreasing cardiac parasite burdens

    2019 | JoVE

RECENT PUBLICATIONS View All Publications >

    Peritoneal Fluid Cytokines Reveal New Insights of Endometriosis Subphenotypes

    2020  |  Nature Cancer

    Trispecific antibodies enhance the therapeutic efficacy of tumor-directed T cells through T cell receptor co-stimulation

    2020  |  Nature Cancer

    Post-hoc assessment of the immunogenicity of three antibodies reveals distinct immune stimulatory mechanisms

    2020  |  Nature Cancer

    TLR4 agonist protects against Trypanosoma cruzi acute lethal infection by decreasing cardiac parasite burdens

    2020  |  Nature Cancer

Get Started

AUTOMATE YOUR FLOW CYTOMETRY WORKFLOW

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FEATURED PRODUCTS

2
Laminar Wash HT2000 System

A reliable and easy-to-use benchtop instrument for high throughput flow cytometry labs.

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Laminar Wash AUTO 1000 System

Complete Automation Solution for Surface Staining and Intracellular Staining for Flow Cytometry.

Laminar Wash AUTO 1000 System
Laminar Wash Mini System

An affordable, benchtop instrument for low-throughput sample preparation for flow cytometry.