COVID-19 & INFECTIOUS DISEASE
Improve biosafety by reducing the generation of aerosols from infectious samples.
Process infectious samples in a biosafety hood.
Increase safety of sample preparation and reduce debris.
See examples of cleaner samples.
Links to useful resources for infectious sample processing.
CHANGE THE WAY YOU PROCESS CELLS TO GET RELIABLY CONSISTENT DATA.
The World Health Organization Laboratory guidance for research on the novel coronavirus SARS-CoV2 is that “ all technical procedures should be performed in a way that minimizes the generation of aerosols and droplets”
|Table 1. Risk prioritization of selected routine laboratory tasks|
|Task or activity||Exposure risk|
|Potential hazard||Likelihood||Consequence||Risk rating|
|Subculturing blood culture bottle||Needle stick — percutaneous inoculation||Likely||Infection; medical treatment||High|
|Aerosols — inhalation||Moderate||Infection; medical treatment||Medium|
|Splash — direct contact with mucous membranes||Moderate||Infection; medical treatment||High|
|Centrifugation||Aerosols — inhalation||Likely||Infection; medical treatment||High|
|Performing Gram stain||Aerosols from flaming slides||Moderate||Colonization; infection||Moderate|
|Preparing AFB smear only||Aerosols from sputum or slide preparation||Likely||Illness; medical treatment; disease||High|
|Performing catalase testing||Aerosols — mucous membrane exposure||Unlikely||Colonization; infection||Low|
|AFB culture work-up||Aerosols — inhalation||Likely||Illness; medical treatment; disease||High|
|Abbreviation: AFB = acid-fast bacillus.|
Curiox Biosystems introduces a new method to prepare immune profiling cell populations within COVID-19 or other infectious disease samples by containing infectious disease blood sample processing within a biosafety cabinet from start to finish, all without the use of a centrifuge, thus eliminating aerosolization of potentially infectious samples.
This new application for infectious disease sample handling combines a new configuration of the Laminar Wash HT2000 system packaged with STEMCELL Technologies EasySep RBC depletion reagent resulting in increased viability and cell retention by reducing stress on already fragile cells that can come from harsh methods such as red blood cell lysis with associated repeated centrifugation steps. This natural and gentle cell prep method using Laminar Wash technology results in superior data and shows improved staining resolution of rare and sensitive immune cellular subsets, like monocytes and neutrophils, that are known to play a crucial role in the immune response against COVID-19 infections.
“The Laminar Washer is safer than flicking (minimal exposure to infectious materials) and requires less space than centrifuges.”
Blanca Ponce-Ngo, Montefiore Medical Center
LAMINAR WASH TECHNOLOGY
Comparison of an immuno-staining workflow for the preparation of a single cell suspension for flow cytometry analysis. Laminar Wash technology is equally effective in washing but without the use of a centrifuge.
BENEFITS OF LAMINAR WASH FOR SINGLE CELL SEQUENCING & PROTEOGENOMICS
The compact size of the Laminar Wash HT2000 or Laminar Wash MINI allow it to easily fit into a biosafety hood. Laminar Wash technology can improve infectious disease sample preparation by:
- Enhancing the safety of the workflow by containing in a hood
- Reduce aerosol production from samples
- Reduce debris, background and clogging of instruments
- Improve resolution and stain index
CENTRIFUGE-LESS RED BLOOD CELL LYSIS AND IMMUNOSTAINING WHOLE BLOOD FOR FLOW CYTOMETRY USING LAMINAR WASH™ TECHNOLOGY
DATA USING THE LAMINAR WASH SYSTEMS
LW is Gentle to Neutrophils and Monocytes
Using 100μL of human whole blood, a comparison study using a 16-color immune profiling panel was conducted to compare LaminarWash to centrifugation protocols. Using Laminar Wash technology, there is increased retention of both the monocyte and neutrophil populations using Laminar Wash utilizing one-step washing, thus minimizing cell perturbations when compared to multiple centrifugation steps.
Better Debris Removal and Cell Retention
Data adapted from the Curiox Biosystems webinar by Dr. Christoph S. Eberle from the Discovery Research Services at Charles River Laboratories, Worcester, MA When performing cell dissociation in tumor bearing tissues, the tissue debris and enzymatic-induced fragments are primary factors affecting cell recovery for tumor infiltrating lymphocytes. For more invasive and immunologically challenging models, like B16F10-inoculated mice, recovery of limited numbers of TILs reduces chances for accurate characterization. The results shown here were demonstrated with an unchallenged mouse sample and the Laminar Wash technology. Laminar washing (with 1,000,000 tumor infiltrate cells) reveals increased viable cell count and reduced debris effects by laminar washing. The cleaner sample preparation increases lymphocyte recovery up to 90.6% as compared to centrifuge-harsh washing of 10.5%.
Data adapted from the Curiox Biosystems webinar by Dr. Christoph S. Eberle from the Discovery Research Services at Charles River Laboratories, Worcester, MA
ALL YOU NEED TO KNOW ABOUT THE LAMINAR
WASH HT2000 SYSTEMS
AUTOMATE YOUR FLOW CYTOMETRY WORKFLOW