HLA CROSSMATCHING

Streamline sample preparation while improving reproducibility.

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Laminar Wash automates repetitive tasks during sample preparation for cross-matching.

Enhance cell retention, reduce hands-on time and improve reproducibility.

See examples of lower CVs and reduced background.

Links to resources and publications.

Overview

CHANGE THE WAY YOU PROCESS CELLS TO
GET RELIABLY CONSISTENT DATA.

FCXM assays, however, typically use a legacy centrifugation method and its associated washing steps when preparing samples. The Laminar Wash technology provides an alternative that offers improved consistency and reduced biohazard aerosolization by eliminating the need of centrifugation. Microplate or sample ‘flicking’ or the use of a pressurized pipette are also eliminated, reducing the risk of cross contamination and user variation.

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“Laminar Wash System Improves Reproducibility and Increases Sensitivity in Flow Cytometric Crossmatches”

Donna Lucas, Senior Manager Immunogenetics and Transplantation Immunology, Johns Hopkins University
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Technology

TRADITIONAL CENTRIFUGATION

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LAMINAR WASH TECHNOLOGY

The demand for cell analysis by flow cytometry is increasing exponentially, particularly for better quantitation and consistency.

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Benefits

BENEFITS OF LAMINAR WASH FOR HLA CROSSMATCHING

Assays for Flow Cytometry Crossmatching require many manual steps and the data must be reliable as transplant patients rely upon it for lifesaving surgeries. Laminar Wash technology benefits FCXM by:

  • Reducing hands-on time and reducing repetitive motion injuries
  • Improving reproducibility between operators
  • Enhancing cell retention
  • Reducing aerosolization of samples
Featured Resource

CENTRIFUGE-LESS RED BLOOD CELL LYSIS AND IMMUNOSTAINING WHOLE BLOOD FOR FLOW CYTOMETRY USING LAMINAR WASH™ TECHNOLOGY

Scientific Data

DATA USING THE LAMINAR WASH SYSTEMS

Reduced B Cell and T Cell Variability

B-cells and T-cell %CV of MFI of negative and positive controls. Data obtained by 3 different operators running samples in duplicates. Laminar Wash improves user-to-user CV and reduces human error.

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Reduced Background without Pronase Treatment

a. Decreased background in pronase-independent B-cell crossmatching of PBL fresh cells with Laminar Wash™. b. ΔMCF was eliminated with Laminar Wash™ in non-pronase-treated B-cells crossmatched with negative control serum.

Recent Publications View All Publications >

    Large scale cytokine profiling uncovers elevated IL12‑p70 and IL‑17A in severe pediatric acute respiratory distress syndrome

    2021 | Scientific Reports

    Primary Human Dendritic Cells and Whole-Blood Based Assays to Evaluate Immuno-Modulatory Properties of Heat-Killed Commensal Bacteria

    2021 | Vaccines

    Single-Cell Transcriptomic Analyses Define Distinct Peripheral B Cell Subsets and Discrete Development Pathways

    2021 | Frontiers in Immunology

    The pleiotropic effects of prebiotic galacto-oligosaccharides on the aging gut

    2021 | Microbiome

Recent Publications View All Publications >

    Large scale cytokine profiling uncovers elevated IL12‑p70 and IL‑17A in severe pediatric acute respiratory distress syndrome

    2021 | Scientific Reports

    Primary Human Dendritic Cells and Whole-Blood Based Assays to Evaluate Immuno-Modulatory Properties of Heat-Killed Commensal Bacteria

    2021 | Vaccines

    Single-Cell Transcriptomic Analyses Define Distinct Peripheral B Cell Subsets and Discrete Development Pathways

    2021 | Frontiers in Immunology

    The pleiotropic effects of prebiotic galacto-oligosaccharides on the aging gut

    2021 | Microbiome

Get Started

AUTOMATE YOUR FLOW CYTOMETRY WORKFLOW

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FEATURED PRODUCTS

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Laminar Wash HT2000

A reliable and easy-to-use benchtop instrument for high-throughput flow cytometry labs.

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Laminar Wash AUTO 1000

Complete Automation Solution for Surface Staining and Intracellular Staining for Flow Cytometry.

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Laminar Wash Mini

An affordable, benchtop instrument for low-throughput sample preparation for flow cytometry.