TUMOR MICROENVIRONMENT

Standardize tumor sample preparation and reduce background for more accurate immunotherapy characterization.

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Achieve cleaner samples with Laminar Wash technology

Enhance cell retention and viability with less debris

See example TILs data using flow cytometry

Publications and links to useful resources

Overview

CHANGE HOW YOU PROCESS CELLS TO GET RELIABLY CONSISTENT DATA.

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“Due to its underlying principle, the Laminar Wash (system) means less mechanical stress on cells and less cell loss from a sample, compared to conventional centrifugation steps.”

Dr. Christoph Eberle, Charles River Laboratories
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Technology

TRADITIONAL CENTRIFUGATION

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LAMINAR WASH TECHNOLOGY

Comparison of an immuno-staining workflow for the preparation of a single cell suspension for flow cytometry analysis. Laminar Wash technology is equally effective in washing but without the use of a centrifuge.

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Benefits

BENEFITS OF LAMINAR WASH FOR TUMOR MICROENVIRONMENT SAMPLES

Tumor microenvironment and TILs isolation are difficult to prepare. Laminar Wash processing yields a clean sample while reducing hands-on time. Laminar Wash technology can:

  • Enhance cell retention
  • Improve reproducibility between operators, time, and locations
  • Improve cell viability and reduce stress on cells
  • Reduce debris, background, artifacts and clogging
  • Offer facile solutions to automate cell prep workflows
Featured Resource

WHITE PAPER: Improved sample preparation method for immuno-oncology studies

Scientific Data

Laminar Wash Technology Improves Debris Removal, TILs Retention, and Resolution Of Subpopulations

Data adapted from the Curiox Biosystems webinar by Dr. Christoph S. Eberle from the Discovery Research Services at Charles River Laboratories, Worcester, MA
Better debris removal and cell retention:

When performing cell dissociation in tumor bearing tissues, the tissue debris and enzymatic-induced fragments are primary factors affecting TILs cell recovery. For more invasive and immunologically challenging models, like B16F10-inoculated mice, recovery of limited numbers of TILs reduces the chances for accurate characterization. Here, an unchallenged mouse sample containing 1e6 TILs was prepared with Laminar Wash technology. Laminar Wash improved viable cell counts and reduced debris, compared to conventional methods. The cleaner sample preparation increases lymphocyte recovery up to 90.6%, compared to centrifuge-harsh washing of 10.5%.

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Laminar Wash Technology Improves Cell Viability

Samples processed with Laminar Wash show higher viability

Splenocytes (a) and (b) and dissociated tumor cells (c) and (d) were washed with either centrifugation (manual 1 and 2) or the Laminar Wash system and viability measurements were compared. Statistical significance is reported among the manual methods vs Laminar Wash: ns = not significant, ** = P<0.01, *** = P<0.001, **** = P<0.0001. The values represent technical triplicates of the samples from an
individual naïve and an individual challenged Balb/c mouse. Manually processed samples were handled by two different analysts..

Figure adapted from Curiox Biosystems and Charles River Laboratories co-authored White Paper

Recent Publications View All Publications >

    Single-Cell Transcriptomic Analyses Define Distinct Peripheral B Cell Subsets and Discrete Development Pathways

    2021 | Frontiers in Immunology

    The pleiotropic effects of prebiotic galacto-oligosaccharides on the aging gut

    2021 | Microbiome

    CTLA-4 blockade drives loss of Treg stability in glycolysis-low tumours

    2021 | Nature

    Peritoneal Fluid Cytokines Reveal New Insights of Endometriosis Subphenotypes

Recent Publications View All Publications >

    Single-Cell Transcriptomic Analyses Define Distinct Peripheral B Cell Subsets and Discrete Development Pathways

    2020  |  Nature Cancer

    The pleiotropic effects of prebiotic galacto-oligosaccharides on the aging gut

    2020  |  Nature Cancer

    CTLA-4 blockade drives loss of Treg stability in glycolysis-low tumours

    2020  |  Nature Cancer

    Peritoneal Fluid Cytokines Reveal New Insights of Endometriosis Subphenotypes

    2020  |  Nature Cancer

Get Started

AUTOMATE YOUR FLOW CYTOMETRY WORKFLOW

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FEATURED PRODUCTS

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Laminar Wash HT2000

A reliable and easy-to-use benchtop instrument for high-throughput flow cytometry labs.

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Laminar Wash AUTO 1000

Complete Automation Solution for Surface Staining and Intracellular Staining for Flow Cytometry.

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Laminar Wash Mini

An affordable, benchtop instrument for low-throughput sample preparation for flow cytometry