Standardize tumor sample preparation and reduce background for more accurate immunotherapy characterization.
Achieve cleaner samples with Laminar Wash technology
Enhance cell retention and viability with less debris
See example TILs data using flow cytometry
Publications and links to useful resources
CHANGE HOW YOU PROCESS CELLS TO GET RELIABLY CONSISTENT DATA.
“Due to its underlying principle, the Laminar Wash (system) means less mechanical stress on cells and less cell loss from a sample, compared to conventional centrifugation steps.”
Dr. Christoph Eberle, Charles River Laboratories
LAMINAR WASH TECHNOLOGY
Comparison of an immuno-staining workflow for the preparation of a single cell suspension for flow cytometry analysis. Laminar Wash technology is equally effective in washing but without the use of a centrifuge.
BENEFITS OF LAMINAR WASH FOR TUMOR MICROENVIRONMENT SAMPLES
Tumor microenvironment and TILs isolation are difficult to prepare. Laminar Wash processing yields a clean sample while reducing hands-on time. Laminar Wash technology can:
- Enhance cell retention
- Improve reproducibility between operators, time, and locations
- Improve cell viability and reduce stress on cells
- Reduce debris, background, artifacts and clogging
- Offer facile solutions to automate cell prep workflows
WHITE PAPER: Improved sample preparation method for immuno-oncology studies
Laminar Wash Technology Improves Debris Removal, TILs Retention, and Resolution Of Subpopulations
Data adapted from the Curiox Biosystems webinar by Dr. Christoph S. Eberle from the Discovery Research Services at Charles River Laboratories, Worcester, MA
Better debris removal and cell retention:
When performing cell dissociation in tumor bearing tissues, the tissue debris and enzymatic-induced fragments are primary factors affecting TILs cell recovery. For more invasive and immunologically challenging models, like B16F10-inoculated mice, recovery of limited numbers of TILs reduces the chances for accurate characterization. Here, an unchallenged mouse sample containing 1e6 TILs was prepared with Laminar Wash technology. Laminar Wash improved viable cell counts and reduced debris, compared to conventional methods. The cleaner sample preparation increases lymphocyte recovery up to 90.6%, compared to centrifuge-harsh washing of 10.5%.
Laminar Wash Technology Improves Cell Viability
Samples processed with Laminar Wash show higher viability
Splenocytes (a) and (b) and dissociated tumor cells (c) and (d) were washed with either centrifugation (manual 1 and 2) or the Laminar Wash system and viability measurements were compared. Statistical significance is reported among the manual methods vs Laminar Wash: ns = not significant, ** = P<0.01, *** = P<0.001, **** = P<0.0001. The values represent technical triplicates of the samples from an
individual naïve and an individual challenged Balb/c mouse. Manually processed samples were handled by two different analysts..
Figure adapted from Curiox Biosystems and Charles River Laboratories co-authored White Paper
ALL YOU NEED TO KNOW ABOUT THE LAMINAR WASH HT2000 SYSTEM
AUTOMATE YOUR FLOW CYTOMETRY WORKFLOW