TUMOR MICROENVIRONMENT

Standardize tumor sample preparation and reduce background for more accurate tumor data.

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Achieve cleaner samples with less debris with Laminar Wash technology

Enhance cell retention and viability.

See examples of TILs data using flow cytometry

Publications and links to useful resources

Overview

CHANGE THE WAY YOU PROCESS CELLS TO
GET RELIABLY CONSISTENT DATA.

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“Due to its underlying principle, the Laminar Wash (system) means less mechanical stress on cells and less cell loss from a sample, compared to conventional centrifugation steps.”

Dr. Christoph Eberle, Charles River Laboratories
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Technology

TRADITIONAL CENTRIFUGATION

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LAMINAR WASH TECHNOLOGY

Comparison of an immuno-staining workflow for the preparation of a single cell suspension for flow cytometry analysis. Laminar Wash technology is equally effective in washing but without the use of a centrifuge.

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Benefits

BENEFITS OF LAMINAR WASH FOR TUMOR MICROENVIRONMENT SAMPLES

Tumor microenvironment and isolation of TILs are difficult to prepare. Laminar Wash processing produces a clean sample at the end of the washing process. Laminar Wash technology can:

  • Enhance cell retention
  • Improve reproducibility between operators, time and locations
  • Improve cell viability and reduce stress on cells
  • Reduce debris, background, artifacts and clogging
Featured Resource

CENTRIFUGE-LESS RED BLOOD CELL LYSIS AND IMMUNOSTAINING WHOLE BLOOD FOR FLOW CYTOMETRY USING LAMINAR WASH™ TECHNOLOGY

Scientific Data

HIGHER RETENTION OF

Laminar Wash Technology Improved Debris Removal And Resulted In Higher Retention Or TILs And A Better Resolution Of Populations
Data adapted from the Curiox Biosystems webinar by Dr. Christoph S. Eberle from the Discovery Research Services at Charles River Laboratories, Worcester, MA Better debris removal and cell retention:

When performing cell dissociation in tumor bearing tissues, the tissue debris and enzymatic-induced fragments are primary factors affecting cell recovery for tumor infiltrating lymphocytes. For more invasive and immunologically challenging models, like B16F10-inoculated mice, recovery of limited numbers of TILs reduces chances for accurate characterization. The results shown here were demonstrated with an unchallenged mouse sample and the Laminar Wash technology. Laminar washing (with 1,000,000 tumor infiltrate cells) reveals increased viable cell count and reduced debris effects by laminar washing. The cleaner sample preparation increases lymphocyte recovery up to 90.6% as compared to centrifuge-harsh washing of 10.5%.

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The Use Of The Laminar Wash Technology Resulted In Higher Cell Viability

Data adapted from the Curiox Biosystems webinar by Dr. Christoph S. Eberle from the Discovery Research Services at Charles River Laboratories, Worcester, MA Better debris removal and cell retention:

Post-dissociation of tumor bearing tissues, the viability of cells is frequently measured and here, presented as the frequency of viable CT26 tumor cells (%parent population between unchallenged (naïve), and anti-mPD-1 challenged). Variability in methods of washing may be impacted by different levels of cell concentration inputs and handling practices. Analysis of samples via manually centrifuge-processed samples by two different analysts and by Laminar Wash showcases the beneficial effect of gentle, reproducible and automated cell washing.

Recent Publications View All Publications >

    Peritoneal Fluid Cytokines Reveal New Insights of Endometriosis Subphenotypes

    Trispecific antibodies enhance the therapeutic efficacy of tumor-directed T cells through T cell receptor co-stimulation

    2020 | Nature Cancer

    Post-hoc assessment of the immunogenicity of three antibodies reveals distinct immune stimulatory mechanisms

    2019 | JoVE

    TLR4 agonist protects against Trypanosoma cruzi acute lethal infection by decreasing cardiac parasite burdens

    2019 | JoVE

Recent Publications View All Publications >

    Peritoneal Fluid Cytokines Reveal New Insights of Endometriosis Subphenotypes

    2020  |  Nature Cancer

    Trispecific antibodies enhance the therapeutic efficacy of tumor-directed T cells through T cell receptor co-stimulation

    2020  |  Nature Cancer

    Post-hoc assessment of the immunogenicity of three antibodies reveals distinct immune stimulatory mechanisms

    2020  |  Nature Cancer

    TLR4 agonist protects against Trypanosoma cruzi acute lethal infection by decreasing cardiac parasite burdens

    2020  |  Nature Cancer

Get Started

AUTOMATE YOUR FLOW CYTOMETRY WORKFLOW

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FEATURED PRODUCTS

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Laminar Wash HT2000

A reliable and easy-to-use benchtop instrument for high-throughput flow cytometry labs.

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Laminar Wash AUTO 1000

Complete Automation Solution for Surface Staining and Intracellular Staining for Flow Cytometry.

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Laminar Wash Mini

An affordable, benchtop instrument for low-throughput sample preparation for flow cytometry