Bead-Based Immunoassays

Reliable Data from Miniaturization
Miniaturization at the 10–15 µl level maintains the statistical analysis with sufficient beads present
Excellent retention of beads, even after extensive washing

According to a publication at Journal of Immunological Methods by Prof. Beaumier at Baylor and Dr. Biswas at Merck, "the DA-Bead method for analysis was comparable to, if not superior to, the conventional method in terms of consistency/precision, accuracy, sensitivity and dynamic range."

It is known that bead-based assays sometimes suffer from bead aggregation with plasma, blood, and other challenging biological samples which affects detectability of proteins and jeopardizes quality of data. Scientific studies have shown that DropArray may reduce bead aggregation and may better permit reading beads individually reporting the signals of target proteins as they are.

The Curiox DA-Bead Plate vs. Traditional Plate Technology
(A) Incubation method principle in traditional plate (left) and DA-Bead Plate (right). Beads are freely floating with shaking in traditional well at 75µl volume vs. beads are fixed in a 15 µl drop in DA-Bead Plate by a magnet below the well and shaken.
(B) Washing method principle in traditional plate (left) and DA-Bead Plate (right). Magnet array with traditional plate either super packs beads at the side of a traditional well or keep beads as a monolayer resulting in aspiration of beads and/or incomplete washing with the nozzle/pipet based dispenser. Conversely, DA-Bead Plate magnet configuration maintains beads as a monolayer (right) resulting in complete washing with gentle laminar flow and minimal residual wash buffer leftover.


More Data Points Using Less Sample and Reagents. Significant Savings.
A 5-fold miniaturization reduces total assay costs by 60–80%
DropArray™ plates allow 5x more wells to be run from a single assay kit
Multiplex bead-based assays are robust and generate highly sensitive data from a variety of samples types. However, these assays are costly and involve expensive reagents and often valuable samples. Curiox’s new DropArray™ plates allow users to analyze the same number of analytes at a fraction of the cost and sample consumption. DropArray™ plates use 5-fold smaller volumes, so the same amount of reagent can produce 5x more data points.

Easy Set Up. Flexible Format.
DropArray™ plates are designed to work with existing workflows
Use your established, standard protocols with DropArray™ plates. The overall workflow remains the same. Available in 96-well format, the plates are offered individually or in bulk to suit your assay needs. Because they are easy to use with standard robotics, therefore there’s no need for additional capital investment. DropArray™ plates are compatible with magnetic beads.

How DA-Bead Plates Stack Up

  DA-Bead Plate (96-well plate) MTP (96-well plate)
Sample volume 10 µl* 50 µl*
Bead volume 5 µl* 25 µl*
Ease of washing Comparable to MTP N/A
No. of wells per kit 480 96
Total reagent cost of one 96-well assay $300 $1500
*These values may vary based on type of a bead-based immunoassay.