Profiling Circulating T cells and Tumor Landscape for Predicting Responses to PD-1 blockade in Patients with Non-Small Cell Lung Cancer
Dutta, N. (2024, September 5). https://gupea.ub.gu.se/handle/2077/81804 Immune checkpoint blockade (ICB) has significantly improved outcomes in non-small cell lung cancer (NSCLC), yet only a subset of patients achieves durable responses. This underscores the need for effective methods to assess ICB efficacy. This thesis investigates the response to PD-1 blockade in NSCLC patients across stages I to […]
Nasal tissue-resident memory CD4+ T cells persist after influenza A virus infection and provide heterosubtypic protection
NimithaR. Mathew, Romain Gailleton, Lydia Scharf, Karin Schön, Anneli Strömberg, Nils Lycke, Mats Bemark, Ka-Wei Tang, Davide Angelettihttps://doi.org/10.1101/2024.07.06.602325 CD4 tissue-resident memory T (CD4 TRM) cells are crucial adaptive immune components involved in preventing influenza A virus (IAV) infection. Despite their importance, their physiological role in the upper respiratory tract, the first site of contact with IAV, remains unclear. Here, we find that, after IAV infection, antigen-specific CD4 TRM persist in the […]
A unified model-based framework for doublet or multiplet detection in single-cell multiomics data
Hu, H., Wang, X., Feng, S., Xu, Z., Liu, J., Heidrich-O’Hare, E., Chen, Y., Yue, M., Zeng, L., Rong, Z., Chen, T., Billiar, T., Ding, Y., Huang, H., Duerr, R. H., & Chen, W. (2024). Nature Communications, 15(1). https://doi.org/10.1038/s41467-024-49448-x Droplet-based single-cell sequencing techniques rely on the fundamentalassumption that each droplet encapsulates a single cell, enabling […]
Protocol to characterize immune cell subpopulations in cerebrospinal fluid of patients with neuroinflammatory diseases using mass cytometry
Gallaccio, G., Wang, M., Schlickeiser, S., Kunkel, D., Böttcher, C., & Fernández-Zapata, C. (2024). STAR Protocols, 5(2), 103038. https://doi.org/10.1016/j.xpro.2024.103038 Phenotypic and compositional changes of immune cells in cerebrospinal fluid (CSF) can be used as biomarkers to help diagnose and track disease activity for neuroinflammatory and neurodegenerative diseases. Here, we present a workflow to perform high-dimensional […]
Reinvigoration of Progenitor-Exhausted CD8 T Cells by anti-CTLA-4 Contributes to the Sustained Activity of Combination Checkpoint Blockade
Wang, Kevin and Coutifaris, Paulina and Brocks, David and Solis, Sabrina and Han, Nicholas and Manne, Sasikanth and Kiner, Evgeny and Sachar, Chirag and George, Sangeeth and Yan, Patrick and Kier, Melanie W. and Laughlin, Amy I. and Kothari, Shawn and Giles, Josephine R. and Mathew, Divij and Ghinnagow, Rheem and Alanio, Cecile and Flowers, […]
Future-Proofing Single Cell Applications
Now Watchinghttps://youtu.be/wL2tsRsL9TA Technology has evolved from those early days of batching thousands of cells together to decrypt the millions of base pairs that make up genetic information. Cellular Indexing of Transcriptomes and Epitopes by sequencing (CITE-seq), brings together surface protein phenotyping (similar to flow cytometry) and single-cell RNA sequencing (scRNA-seq). This is achieved by using […]
Longitudinal single-cell analysis of SARS-CoV-2–reactive B cells uncovers persistence of early-formed, antigen-specific clones
Scharf, L., Axelsson, H., Emmanouilidi, A., Mathew, N. R., Sheward, D. J., Leach, S., Isakson, P., Smirnov, I. V., Marklund, E., Miron, N., Andersson, L., Gisslén, M., Murrell, B., Lundgren, A., Bemark, M., & Angeletti, D. (2022). JCI Insight, 8(1). https://doi.org/10.1172/jci.insight.165299 Understanding persistence and evolution of B cell clones after COVID-19 infection and vaccination is […]
Using high throughput technologies to understand heterogeneous drug response
GE Boyle (2023), University of Washington Heterogeneity is an inherent quality of biological systems, and is one of the properties that facilitates adaptation of organisms to new environments and stimuli. However, the scientific method relies on probabilistic reasoning wherein sample measurements are assumed to represent the population as a whole. In situations where this assumption […]
Overcoming challenges of Nuclei and Cell Preparation: A scientific perspective by S2 genomics and Curiox Story
Now Watchinghttps://youtu.be/inLymDAn4EI Single-cell transcriptome analysis has been extensively applied in humans and animal models to uncover gene expression heterogeneity between the different cell types of a tissue or an organ. Upstream processing may introduce inherent challenges that mask true analysis and increase user variability. Here we present a simple, yet combinatorial strategy from buffer and […]
Laminar Wash MINI000 Virtual Demonstration with CITE-Seq Protocol
Now Watchinghttps://www.youtube.com/watch?v=HrvllFVj3K0 This video demonstrates the daily operation and utility of the Laminar WashTM MINI1000 system, hosted by Curiox Biosystems scientist Amira Amilia, and features a common protocol example in setting up a CITE-Seq (Cellular Indexing of Transcriptomes and Epitopes by Sequencing) staining assay. Comprised of a proprietary 16-well “wall-less” strip and a fluidics instrument, the MINI1000 is the […]