Protocol to characterize immune cell subpopulations in cerebrospinal fluid of patients with neuroinflammatory diseases using mass cytometry
Gallaccio, G., Wang, M., Schlickeiser, S., Kunkel, D., Böttcher, C., & Fernández-Zapata, C. (2024). STAR Protocols, 5(2), 103038. https://doi.org/10.1016/j.xpro.2024.103038 Phenotypic and compositional changes of immune cells in cerebrospinal fluid (CSF) can be used as biomarkers to help diagnose and track disease activity for neuroinflammatory and neurodegenerative diseases. Here, we present a workflow to perform high-dimensional […]
A novel mass cytometry protocol optimized for immunophenotyping of low-frequency antigen- specific T cells
Balz, K., Grange, M., Pegel, U., Karamya, Z. A., Mello, M., Zhou, X., Berger, T., Bloch, K., Dunham, D., Chinthrajah, S., Nadeau, K., Luche, H., & Skevaki, C. (2024b). Frontiers in Cellular and Infection Microbiology, 13. https://doi.org/10.3389/fcimb.2023.1336489 Understanding antigen-specific T-cell responses, for example, following virus infections or allergen exposure, is of high relevance for the […]
Surfaceome mapping of primary human heart cells with CellSurfer uncovers cardiomyocyte surface protein LSMEM2 and proteome dynamics in failing hearts
READ NATURE Cell surface proteins (surfaceome) play a significant role in the development of novel target therapies. Therefore, a comprehensive surfaceome profiling approach is necessary to identify and quantify specific markers for effective therapeutic targeting. The challenge most developers face is the lack of data due to complex methodologies, rare cell types and/or small number […]
Using high throughput technologies to understand heterogeneous drug response
GE Boyle (2023), University of Washington Heterogeneity is an inherent quality of biological systems, and is one of the properties that facilitates adaptation of organisms to new environments and stimuli. However, the scientific method relies on probabilistic reasoning wherein sample measurements are assumed to represent the population as a whole. In situations where this assumption […]
Nothing Mundane, Be Like Jane!
Now Watchinghttps://youtu.be/3LeDxQ5YeRo
AUTO1000: Automate Your Cell Staining Workflow
The Curiox Laminar Wash AUTO 1000 System completely automates the staining and washing workflow, eliminating a major source of variability and enabling process standardization. The AUTO 1000 system offers several key benefits to research and shared resource laboratories in cell and gene therapy discovery and process development, antibody screening, flow core labs, and other applications that rely on cell-based assays.
Enabling Single-cell Multiomics and Cell Hashing in Studies with Limited Numbers of Cells
Now Watchinghttps://youtu.be/LagyuoWvELo Our October 19, 2021 webinar featured University of Gothenburg Associate Professor of Immunology Mats Bemark, Ph.D. and Curiox Biosystems Marketing & Content Manager, Geoffrey Feld, Ph.D. Dr. Bemark studies mucosal immunology, with particular focus on the gut and gut-related infections like cholera, and recently has been working on COVID-19 with human severe disease samples. His […]
Benefits of Using the Curiox Laminar Wash System in Mass Cytometry Experiments
Now Watchinghttps://www.youtube.com/watch?v=qOP26iVEnQs Description Jorgen Adolfsson is head of the Mass Cytometry Unit at Linköping University in Sweden, which is part of the SciLifeLab National Infrastructure. In this webinar Jorgen speaks about his experience using the Laminar Wash system. Jorgen describes reduction of antibody usage by ~50% with preserved signal and reproducibility. Laminar Wash technology has […]