Webinar On-Demand: Shift the Paradigm to Centrifuge-less Immuno-staining of Cells for Superior Viability, Recovery & Data

COVID-19 & INFECTIOUS DISEASE
Reduce aerosol generation from infectious samples to improve biosafety.

Technology

Process infectious samples in a biosafety hood.

Benefits

Prepare samples safely while reducing debris.

Scientific Data

See examples of cleaner samples.

Resources

Links to useful resources for infectious sample processing.

Overview

CHANGE THE WAY YOU PROCESS CELLS TO GET RELIABLY CONSISTENT DATA

The World Health Organization Laboratory guidance for research on the novel coronavirus SARS-CoV2 states, “all technical procedures should be performed in a way that minimizes the generation of aerosols and droplets.”

Table 1. Risk prioritization of selected routine laboratory tasks
Task or activity Exposure risk      
Potential hazard Likelihood Consequence Risk rating
Subculturing blood culture bottle Needle stick — percutaneous inoculation Likely Infection; medical treatment High
Aerosols — inhalation Moderate Infection; medical treatment Medium
Splash — direct contact with mucous membranes Moderate Infection; medical treatment High
Centrifugation Aerosols — inhalation Likely Infection; medical treatment High
Performing Gram stain Aerosols from flaming slides Moderate Colonization; infection Moderate
Preparing AFB smear only Aerosols from sputum or slide preparation Likely Illness; medical treatment; disease High
Performing catalase testing Aerosols — mucous membrane exposure Unlikely Colonization; infection Low
AFB culture work-up Aerosols — inhalation Likely Illness; medical treatment; disease High
Abbreviation: AFB = acid-fast bacillus.

Curiox Biosystems introduces a new, safer method for preparing potentially or known infectious blood samples for immune cell profiling without a centrifuge, eliminating aerosolization of potentially infectious samples. The method combines a new configuration of the Laminar Wash HT2000 system packaged with STEMCELL Technologies EasySep RBC depletion reagent to enable blood sample processing within a biosafety cabinet from start to finish.

This natural and gentle cell prep method relying on Laminar Wash technology improves viability, retention, and staining resolution of rare and sensitive immune cell subsets known to play crucial roles in the immune response against COVID-19 infections, like monocytes and neutrophils. Improvements in data quality due to reduced stress are evident even on fragile cells resulting from harsh methods such as red blood cell lysis that relies upon repeated centrifugation steps.

“The Laminar Washer is safer than flicking [minimizing exposure to infectious materials] and requires less space than centrifuges.”
Blanca Ponce-Ngo, Montefiore Medical Center

TRADITIONAL

CENTRIFUGATION

VS

LAMINAR FLOW

TECHNOLOGY

Comparison of an immuno-staining workflow for the preparation of a single cell suspension for flow cytometry analysis. Laminar Wash technology is equally effective in washing but without the use of a centrifuge.

Benefits
LAMINAR WASH FOR SINGLE CELL SEQUENCING & PROTEOGENOMICS

The compact sizes of the Laminar Wash HT2000 and Laminar Wash MINI means easy containment in a biosafety cabinet. Laminar Wash technology can improve infectious disease sample preparation by:

  • Enhancing workflow safety through biosafety cabinet containment
  • Reducing aerosol production from samples
  • Reducing debris, background, and instrument clogging
  • Improving resolution and stain index
Featured Resource
Severe COVID-19 infection is associated with aberrant cytokine production by infected lung epithelial cells rather than by systemic immune dysfunction
Scientific Data
DATA USING THE LAMINAR WASH SYSTEMS
LW is Gentle on Both Neutrophils and Monocytes

Laminar Wash technology was compared to centrifugation protocols in a 16-color immune profiling panel using 100μL of whole human blood. One-step washing using Laminar Wash minimized cell perturbations compared to multiple centrifugation steps, increasing retention of both the monocyte and neutrophil populations.

Better Debris Removal and Cell Retention

When performing cell dissociation in tumor-bearing tissues, tissue debris and enzymatic-induced fragments are primary factors affecting recovery of tumor infiltrating lymphocytes (TILs). For more invasive and immunologically challenging models, like B16F10-inoculated mice, recovery of limited numbers of TILs hinders accurate characterization. Here, Laminar Wash technology was compared to conventional methods for preparing an unchallenged mouse sample containing ~1,000,000 tumor infiltrate cells. Laminar Wash improved viable cell count and reduced debris formation. The cleaner sample preparation increased lymphocyte recovery up to 90.6%, compared to 10.5% for the centrifuge.
Data adapted from the Curiox Biosystems webinar by Dr. Christoph S. Eberle, Discovery Research Services at Charles River Laboratories, Worcester, MA

FEATURED RESOURCES
ALL YOU NEED TO KNOW ABOUT THE LAMINAR WASH™ HT2000 SYSTEM
Walk-Away Automation of Cell Immunostaining Assays by Centrifuge-Less Laminar Wash™
Centrifuge-less Whole Blood Staining with Laminar Wash in a Biosafety Cabine
Integrating Centrifuge Independent and Time Efficient Automated Washing in Flow Cytometry Crossmatching
RELATED PRODUCTS AND ACCESSORIES
LAMINAR WASH HT2000
A reliable and easy-to-use benchtop instrument for high throughput flow cytometry labs.
LAMINAR WASH AUTO 1000
Complete Automation Solution for Surface Staining and Intracellular Staining for Flow Cytometry.
LAMINAR MINI SYSTEM
An affordable, benchtop instrument for low-throughput sample preparation for flow cytometry.
Get Started
AUTOMATE YOUR FLOW CYTOMETRY WORKFLOW