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DROPARRAY BEAD PLATES
Reduce sample preparation, improve data quality and biosafety

Wall-less plate technology for miniaturizing bead-based immunoassays enabling small sample volumes (2-5𝛍l), reducing bead clumping, and saving reagents and antibodies.

Miniaturization with the DropArray-Bead plates reduces sample volume and bead consumption by 5X and decreases assay costs by >60%. Laminar flow washing is efficient and limits bead aggregat

What the DropArray Bead Plates deliver:
  • Sample savings
  • Ability to utilize existing workflows
  • Reduced cost of running multiplex immunoassays
  • Excellent retention of beads for accurate results
TECHNOLOGY OVERVIEW
SEE THE DROPARRAY BEAD PLATES IN ACTION
Miniaturization with the DA-Bead plates reduces sample volume and bead consumption by 5X and decreases assay costs by >60%. Laminar flow washing is efficient and limits bead aggregation.
“The DA-Bead method for analysis was comparable to, if not superior to, the conventional method in terms of consistency/precision, accuracy, sensitivity and dynamic range.”
Prof. Beaumier at Baylor and Dr. Biswas at Merck
THE CURIOX DA-BEAD PLATE

VS

TRADITIONAL PLATE TECHNOLOGY

Washing Stations accept DropArray™ DA-Bead and DA-HCS plates into a liquid-tight chamber formed with a clamping mechanism. After an aqueous washing buffer is automatically added, the station gently shakes the microplate to facilitate washing with minimal loss of cells. The station then tilts the microplate, using gravity to drain the excess washing buffer from the planar surface, avoiding the harsh, high-velocity liquid exchange associated with traditional washers and microplates. Buffer is added at a very low velocity, preventing cell and bead loss. In addition, there is a magnet array embedded in the plate holder for bead-based immunoassays. This helps retain magnetic beads on the microplate upon washing. Despite the low velocity, plate processing time is comparable to traditional washers because the entire plate is treated at once. After the microplate is removed from the station, the plate is ready for accepting the next set of reagents.

 

DROPARRAY™ PLATES WORK WITH EXISTING WORKFLOWS
Use your established, standard protocols with DropArray™ plates. The overall workflow remains the same. Available in 96-well format, the plates are offered individually or in bulk to suit your assay needs. Because they are easy to use with standard robotics, therefore there’s no need for additional capital investment. DropArray™ plates are compatible with magnetic beads.
HOW DA-BEAD PLATES STACK UP
 

DA-Bead Plate (96-well plate)

MTP (96-well plate)

Sample volume

10µl*

50µl*

Bead volume

5µl*

25µl**

Ease of washing

Comparable to MTP

N/A

No. of wells per kit

480

96

Total reagent cost of one 96-well assay

$300

$1500

*These values may vary based on type of bead-based immunoassay.

DATA USING THE DROPARRAY SYSTEM
Peritoneal Fluid Cytokine Multiplex Analysis
When performing cell dissociation in tumor bearing tissues, the tissue debris and enzymatic-induced fragments are primary factors affecting cell recovery for tumor infiltrating lymphocytes. For more invasive and immunologically challenging models, like B16F10-inoculated mice, recovery of limited numbers of TILs reduces chances for accurate characterization. The results shown here were demonstrated with an unchallenged mouse sample and the Laminar Wash technology. Laminar washing (with 1,000,000 tumor infiltrate cells) reveals increased viable cell count and reduced debris effects by laminar washing. The cleaner sample preparation increases lymphocyte recovery up to 90.6% as compared to centrifuge-harsh washing of 10.5%.
Multiplex Immunoassay for a Cytokine Re-stimulation Assay

Cytokine profiles of BALB/c mice immunized with rAs37 formulated with Alhydrogel, MPL, and AddaVax. Cytokine levels were determined in supernatants of splenocytes after being re-stimulated with rAs37 (25 μg/ml) for 48 hours. Cytokine values measured in unstimulated samples were subtracted from the cytokine values from the corresponding rAs37 re-stimulated samples. Data are presented as means and individual values for each group (n = 5). *p< 0.05, **p< 0.01.

https://doi.org/10.1371/journal.pntd.0008057.g005

ACCESSORIES AND COMPONENTS

96-well DA-Bead Plate

96-CC-BD-05

384-well DA-Bead Plate

384-CC-BD-05

DropArray™ Bead Reading Fluid

BD-RD-01-01

FEATURED RESOURCES
ALL YOU NEED TO KNOW ABOUT THE DROPARRAY™ SYSTEM
Bead-based Immunoassays with DA-Bead Plate: Milliplex Assays
Browse the latest DropArray publications
Transferring Luminex® cytokine assays to a wall-less plate technology: Validation and comparison study with plasma and cell culture supernatants.
RELATED PRODUCTS AND ACCESSORIES
DROPARRAY INSTRUMENTS
Reliable Multiplex Immunoassay Data from Miniaturization
DROPARRAY BEAD PLATES
Wall-less plate technology for miniaturizing bead-based immunoassays enabling small sample volumes
LAMINAR MINI SYSTEM
An affordable, benchtop instrument for low-throughput sample preparation for flow cytometry.
Interested?
GET IN TOUCH TO FIND OUT HOW THE LAMINAR WASH SYSTEMS CAN IMPROVE YOUR WORKFLOW