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What are the major differences between the Laminar Wash™ plate and a traditional microplate?
The Laminar Wash plates are flat-bottom and wall-less with dual satellites, where the wells are hydrophobic. When liquid dispensed onto the wells, it forms droplets that remain in a fixed position from the surface tension created from the hydrophobic surroundings.
How do the cells stay in the well and not get washed away with the laminar flow created by the dual-nozzle action?

During incubation, the cells settle to the bottom of the well without any physical attachment. Once the plate is loaded in the washer, two separate nozzles are lower towards the satellites in each well – one that dispenses buffer and the other one that aspirates. The laminar velocity is high at the top of the well, whereas the velocity at the bottom, where the cells are located, is close to zero.

Does using the Laminar Wash plate require special skills as it has no walls?
Using the Laminar Wash plates is easy and it does not require any special skills, plus they the plates are compatible with robotic arms.
How much wash buffer and washing time are needed to wash plates on the Laminar Washers?
The HT2000 Washer requires ~200 ml for priming the MINI1000 Washer ~50 ml. Both washers use ~65 ml for a 1x washing cycle, and a typical 12x wash cycle needs ~4 minutes when set to the lowest speed (5 µl/s).
What is the minimum time required for cells to settle during the incubation time?
The minimum time for optimized cell recovery is recommended at 15 minutes. The addition of 15 to 25 minutes of incubation time reduces the loss of cells.
Can we use the Laminar Wash plates for direct acquisition in flow cytometers with 96-well loaders?
Yes. The Laminar Wash plates are compatible with most flow cytometers with 96-well loaders.
We are interested in rare cells and events and usually need larger volumes. What is the maximum cell number and volume of reagent per well of a Laminar Wash plate?
In the Laminar Wash plates you can process up to 10 million cells per well, and each well can hold up to 70 µl of reagent, and this volume can increase to 150 µl by using a simple accessory.
Can we perform fixing, permeabilization, and intra-cellular staining on the Laminar Wash plate?
Yes, per your usual protocol the fixing, permeabilization, and staining can be performed all on the Laminar Wash plate.
Do I need to run a full plate or can I run partial plates or re-use the plate?
The plate can be run in full or partially. If only a few wells are used, the plate can be stored at 4°C for up to 3 weeks, and the remainder of the wells can be used later. However, wells cannot be reused and caution must be taken to document the wells that have been used.
What is the typical volume of a well and how many cells can it contain?
The Laminar Wash™ plate can hold up to 10 million cells and up to 70 µl of reagents. The optimum performance in the 100,000 – 3,000,000 cells/well and the reagent volume can be increased to 150 µl by using an adaptor.
Can the Laminar Wash plates process incubations at different temperatures?
Yes. The samples in the Laminar Wash™ plates can be incubated in a range of 2˚C -80˚C.
Can we program the leftover volume after washing?
No. The leftover volume per well is always 25 µl.
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