The Laminar Wash plates are flat-bottom and wall-less with dual satellites, where the wells are hydrophobic. When liquid dispensed onto the wells, it forms droplets that remain in a fixed position from the surface tension created from the hydrophobic surroundings.
During incubation, the cells settle to the bottom of the well without any physical attachment. Once the plate is loaded in the washer, two separate nozzles are lower towards the satellites in each well – one that dispenses buffer and the other one that aspirates. The laminar velocity is high at the top of the well, whereas the velocity at the bottom, where the cells are located, is close to zero.
The HT2000 Washer requires ~200 ml for priming the MINI1000 Washer ~50 ml. Both washers use ~65 ml for a 1x washing cycle, and a typical 12x wash cycle needs ~4 minutes when set to the lowest speed (5 µl/s).
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The minimum time for optimized cell recovery is recommended at 15 minutes. The addition of 15 to 25 minutes of incubation time reduces the loss of cells.
In the Laminar Wash plates you can process up to 10 million cells per well, and each well can hold up to 70 µl of reagent, and this volume can increase to 150 µl by using a simple accessory.
The plate can be run in full or partially. If only a few wells are used, the plate can be stored at 4°C for up to 3 weeks, and the remainder of the wells can be used later. However, wells cannot be reused and caution must be taken to document the wells that have been used.
The Laminar Wash™ plate can hold up to 10 million cells and up to 70 µl of reagents. The optimum performance in the 100,000 – 3,000,000 cells/well and the reagent volume can be increased to 150 µl by using an adaptor.
