Cell Processing for Flow Cytometry

Change the way you process cells to get reliably consistent data.

Laminar Wash technology is for scientists who believe that quantitative flow cytometry is critical to cell therapy, immuno-oncology, and other fields where cell analysis is important.

The demand for cell analysis by flow cytometry is increasing exponentially, particularly for better quantitation and consistency. How can we meet this challenging demand? Well, one way is to hire more scientists and enforce SOPs strictly. Many experts in the field say that this won’t be a solution – even worse as? since? we have a shortage of experienced scientists in flow cytometry and frequent turn-over.

If you’re interested in obtaining the most reproducible data among users and locations, then Laminar Wash technology is for you.

Comparison of an immunostaining workflow for the preparation of cells suspension for analysis by flow cytometry. Laminar Wash technology is equally as effective in washing but without use of a centrifuge.
Affordable and simple automation of sample prep by Laminar Wash technology approach offers many benefits. In cell therapy, we can achieve:
  • Consistency, precision of immune profiling to be the key to the success of therapy
  • Precise and reproducible quantitation leading to biomarkers discovery? Identification? and predicting the success of cell expansion and therapy as early as possible

Operational benefits

  • Capability in accommodating a wide range of demands of cell analysis, particularly in combination with automated data analysis
  • Significant time saving of 70~90 % in processing cells allowing scientists to spend more time on analysis and science
  • Virtual guarantee in consistency and reproducibility
  • Minimal training upon turnover of staff or transfer of assays between departments and sites

Benefits of Laminar Wash process

  • A wide range of cell numbers at 1 cell – 10 million cells per well
  • No stress on cells from centrifugation and improved viability
  • Reduced debris and aggregation of cells
  • Cleaner and faster washing

Laminar Wash™ enables distinct identification of cell populations.

Laminar WashTM enables distinct identification of cell population

Scatterplot of CD20-BV785 versus (empty channel) BV421 of PBMCs processed either with centrifuge or Laminar Wash™. In cells processed by Laminar Wash™ , there is clear separation of positive and negative population due to higher median staining of CD20-expressing cells, compared to cells processed by centrifuge.

Example of Improving Consistency by Automating Only the Wash Step
Laminar WashTM enables distinct identification of cell population

After adoption of the Laminar Wash technology they experience low CVs in populations between operators and between experimental days.

Improved enumeration of γδ T Cells with Laminar Wash™ in whole blood assay

  • Better cell retention of live cells
  • Higher stain index of VD2 marker (also CD4 and other markers)
  • Higher enumeration of rare GDT cells