Laminar Wash™ AUTO 1000 System
Accelerate high-throughput flow and mass cytometry workflows with fully automated sample preparation.
The Laminar Wash AUTO 1000 System eliminates time-consuming and labor-intensive manual pipetting and handling by automating the complete surface and intracellular staining workflow for flow and mass cytometry. You get all the benefits of laminar washing in a completely automated system.
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What the AUTO 1000 delivers:
Laminar Washing Technology
Gentle cell washing in a 96-well format
Optimized Deck Layout
A completely contained system for washer maintenance plates, washer load tray, heater/shaker, cooling device, reagent/buffer plates, input/output stacks and tips.
Laminar Wash AUTO Software
The user-friendly software interface allows sample-specific modifications to methods and comes with pre-programmed protocols to ensure user consistency.
- The same data quality, cell retention, cell viability, and reproducibility benefits of the HT2000 and Mini systems
- A pre-programmed, ready-to-use solution covering the entire sample prep workflow
- An intuitive user interface that is easily programmed for those new to automation as well as automation experts
- Electronic files that can track and trace samples
- The reduction in operator-to-operator variability that automation provides
An innovative design delivers better data
The power of Curiox’s laminar cell washing technology lies in the unique design of the sample plate. Each plate contains an array of hydrophilic spots that suspension cells can rest on via gravity. The material surrounding each spot is highly hydrophobic, acting as an impenetrable wall that separates your samples.
Upon entry into the instrument, two nozzles descend onto each side of every spot, one to dispense wash buffer and the other to aspirate the buffer and debris away.
Each spot can wash as many as 10 million cells down to a single cell without the mechanical stress and cell losses associated with centrifugation.
See how dye is washed away from green fluorescent polystyrene beads during 2 minutes of 7 aspiration and dispense wash cycles. At the end of the cycle, the dye is gone but the polystyrene beads remain in the spot.
Automated laminar washing technology delivers good consistency and increased retention of low input T cell numbers from freshly thawed PBMC
Figure 1. Improved retention compared to centrifugation. When processing a sample with a lower cell count (10,000 - 100,000 cells), Laminar Washing (datapoint in Red) led to retention of more cells than centrifugation (datapoint in Black). The cell count was compared between each additional cycle of Laminar Washing (9 rounds of 60uL wash buffer exchange) and 1 spin wash cycle.