Laminar Wash™ AUTO 1000 System

Accelerate high-throughput flow and mass cytometry workflows with fully automated sample preparation.

The Laminar Wash AUTO 1000 System eliminates time-consuming and labor-intensive manual pipetting and handling by automating the complete surface and intracellular staining workflow for flow and mass cytometry. You get all the benefits of laminar washing in a completely automated system.

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What the AUTO 1000 delivers:
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Laminar Washing Technology

Gentle cell washing in a 96-well format

Optimized Deck Layout

A completely contained system for washer maintenance plates, washer load tray, heater/shaker, cooling device, reagent/buffer plates, input/output stacks and tips.

Laminar Wash AUTO Software

The user-friendly software interface allows sample-specific modifications to methods and comes with pre-programmed protocols to ensure user consistency.

  • The same data quality, cell retention, cell viability, and reproducibility benefits of the HT2000 and Mini systems
  • A pre-programmed, ready-to-use solution covering the entire sample prep workflow
  • An intuitive user interface that is easily programmed for those new to automation as well as automation experts
  • Electronic files that can track and trace samples
  • The reduction in operator-to-operator variability that automation provides

Technology Overview

See the Laminar Wash AUTO 1000 in action.

Achieve truly high throughput suspension cell sample preparation

The Laminar Wash AUTO 1000 is a completely hands-off automated platform to dispense, fix/permeabilize, stain, wash, and transfer samples for flow and mass cytometry.

The system comes ready to use with a pre-programmed sample preparation workflow. Automation novices and experts alike can easily program a custom workflow using the intuitive user interface.
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“The Laminar Wash’s performance allows us to get very tight control over populations with very tight CV’s largely due to the fact of the clear separations between populations. The maintained ability to detect rare populations is important as we detect the next round of cell therapy products.”

Arnaud Colantonio, PhD.
Associate Director of Analytical Development at AdiCet Bio,
now with Kite Pharma

How Our Laminar Flow Technology Works

Compared to a traditional, centrifuge-driven cell washing workflow, laminar cell washing is simpler and faster. With the AUTO 1000, you can prepare 16 samples in just 6 minutes.

An innovative design delivers better data

The power of Curiox’s laminar cell washing technology lies in the unique design of the sample plate. Each plate contains an array of hydrophilic spots that suspension cells can rest on via gravity. The material surrounding each spot is highly hydrophobic, acting as an impenetrable wall that separates your samples.

Upon entry into the instrument, two nozzles descend onto each side of every spot, one to dispense wash buffer and the other to aspirate the buffer and debris away.

Each spot can wash as many as 10 million cells down to a single cell without the mechanical stress and cell losses associated with centrifugation.

dye washing

See how dye is washed away from green fluorescent polystyrene beads during 2 minutes of 7 aspiration and dispense wash cycles. At the end of the cycle, the dye is gone but the polystyrene beads remain in the spot.

Scientific Data

Auto 1000 Cell Retention Data

Automated laminar washing technology delivers good consistency and increased retention of low input T cell numbers from freshly thawed PBMC

Figure 1. Improved retention compared to centrifugation. When processing a sample with a lower cell count (10,000 - 100,000 cells), Laminar Washing (datapoint in Red) led to retention of more cells than centrifugation (datapoint in Black). The cell count was compared between each additional cycle of Laminar Washing (9 rounds of 60uL wash buffer exchange) and 1 spin wash cycle.

Simple and straightforward reproducibility with Laminar Wash Automation

Complement-dependent cytotoxic crossmatch (CDCXM) and flow cytometric crossmatch (FXCM) are both common methods to predict donor-recipient compatibility – the potential success of an organ transplant. FCXM assays are more widely applied across human leukocyte antigen (HLA) labs.  FXCM utilizes the use of multiple washing steps, serum treatment and secondary antibody used. Results between the centrifugation-based and Laminar Washing methods within 8 replicates and 3 test sera and 2 controls show average CVs of 12.4% vs 7.9% respectively. With Laminar Wash technology, greater consistency was seen on the Median Channel Fluorescence values gated on B-cells. The ratio of positive crossmatch yielded a higher shift while maintaining a lower MCF value for the negative background. This is attributed by a superior wash and accuracy in matrix serum handling with primary patient cells.

Technical Specifications
Voltage Requirements 100-240V
Power Consumption +42V/5.0A
Dimensions 88 cm H x 27.9 cm W x 135.9 cm D
34.6 in H x 11 in W x 53.5 in D
Weight 120kg (264.5 lbs)
Accessories and Components
Laminar Wash™ AUTO1000 Station Standard Configuration Auto-1000-96-01
Laminar Wash™ 96-well plate (with a regular lid) 96-DC-CL-05
HEPA Filter AU-HE-01-01
Laminar Wash™ 96 well grid (BVL) DC-GR-96-05
FEATURED RESOURCES

All you need to know about the Laminar Wash AUTO 1000 System

Related Products and Accessories

Laminar-wash-plates-1
Laminar Wash Plates

96 well plates for use with the HT2000 and Auto 1000 Systems

2@2x
Laminar Wash HT2000

A reliable and easy-to-use benchtop instrument for high throughput flow cytometry labs.

3@2x
Laminar Wash Mini

An affordable, benchtop instrument for low-throughput sample preparation for flow cytometry

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