LAMINAR WASH™ DIRECT READING GRID (DRG)
Introducing the all-new Direct Reading Grid (DRG), a volume adaptor designed for YOUR flow cytometer
What the LW DIRECT READING GRID (DRG) delivers:
Inclusive flow cytometer sample preparation workflows
The DRG is meant for direct acquisition on flow cytometers. A list of validated cytometers for use with the DRG and tested parameter settings can be found below.
The acquisition parameters should be used as directions and may require optimization with your specific application and cytometer. For use on cytometers other than those validated, please reach out to your regional Field Application Scientist for assistance.
Listed Cytometers are from the Following:
- https://www.thermofisher.com/order/catalog/product/A42973#/A42973
- https://www.beckman.com/flow-cytometry/research-flow-cytometers/cytoflex
- https://www.bdbiosciences.com/en-sg/products/instruments/flow-cytometers/research-cell-analyzers/facscelesta
- https://www.bdbiosciences.com/en-sg/products/instruments/flow-cytometers/research-cell-analyzers/bd-facsymphony-a5
- http://cytekbio.com/pages/aurora
Technology Overview
DO EVEN MORE WITH THE DIRECT READING GRID
The DRG is a disposable volume adaptor designed to sit on top the Laminar Wash™ Plate forming physical walls around each well to increase the total holding volume of the plate for direct acquisition of samples for flow cytometry and other downstream applications. The DRG can be acquired stably on closed flow cytometer up to 2 -3 hours (for more observations, please check with Curiox Application Support.
Waste containment: regular lab requirements for labware
Dimensions: 124 mm in length, 82 mm in width and 12 mm in height
Storage: Store the DRG in a cool and dry place.
Important Notes:
- One-Time Use
- The Direct Reading Grid is for use with the Laminar Wash™ 96-well plate only
- The DRG is not prescribed for reagent incubation, or for use inside the Curiox Laminar Wash™ station
COMES IN TWO CONFIGURATIONS. FIND WHICH ONES WORK FOR YOU.
- Manual Compatible (LEFT)
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- Gripper added on either side
- Automation Compatible (RIGHT)
- No Gripper added on either side
If you need assistance determining which configuration is right for your system, our team of experts are here to help.
TAKE A LOOK AT THE EASY TO USE AND RELIABLE DIRECT READING GRID
In this video provides walk-throughs on pairing the Direct Reading Grid (DRG) with the Laminar Wash™ (LW) Plate to create the DRG & LW Plate Unit as well as pipetting, handling, transferring, and Big Bear Vortexing with the DRG and LW Plate Unit.
CENTRIFUGE-LESS CELL PROCESSING FOR FLOW CYTOMETRY ASSAYS
Traditional centrifuge-based preparation of cells are time consuming, labor intensive, waste expensive reagents, and stress cells. There is significant cell loss and incomplete washing resulting in problems with gating and cell segmentation.
Scientific Data
The Direct Reading Grid enables direct flow cytometer acquisition from the Laminar Wash™ Plate for better data and efficient sample preparation workflows
The Direct Reading Grid + Laminar Wash™ Plate create a stable and reliable unit with increased volume capacity per well for direct cytometer acquisition delivering consistent and reproducible data.
Demonstrated here is the utilization of the DRG for sample direct acquisition from the Laminar Wash™ 96 well plate with the BD FACSCelesta with HTS flow cytometer. Presented at the highest specified vortexing volume of 250μL, vortexing on the plate shaker, and alternating plating of samples (HL-60 cell line: 250,000 cells/well and Precision Counting Beads: 2μL/well), and reagents (mouse anti-human CD45 and 2%FBS/PBS) for detection of cross-contamination between the wells. Enumeration of events in all 96 wells on two unique LW96+DRG units showed no observable contamination of samples across wells. Analysis of the fluorophore on the cells also revealed that the fluorophore-bound cells were filial to their respective wells, indicating that there was no reagent or sample leakage. This was corroborated by visual observation of the plate and DRG during and after use.
How Laminar Wash™ Works
Enhanced Cell Recovery post cell sorting for lower cell numbers
The power of Curiox’s laminar cell washing technology lies in the unique design of the 96-sample plate. Each plate contains an array of hydrophilic spots that suspension cells can rest on via gravity. The material surrounding each spot is highly hydrophobic, acting as an impenetrable wall that separates your samples.
Upon entry into the instrument, two nozzles descend onto each side of every spot, one to dispense wash buffer and the other to aspirate the buffer and debris away.
Each spot can wash as many as 10 million cells down to a single cell without the mechanical stress and cell losses associated with centrifugation.
Ordering Information
The DRG is available in two configurations:
- Manual Compatible DRG (DC-GR02-96-M): Gripper added on wither side
- Automation Compatible DRG (DC-GR02-96-A): No gripper added on either side
Related Products and Accessories
Laminar Wash HT2000
A reliable and easy-to-use benchtop instrument for high throughput flow cytometry labs.
Laminar Wash Auto 1000
Accelerate high-throughput flow and mass cytometry workflows with fully automated sample preparation.
