Laminar Wash™ HT2000 System
Achieve higher throughputs and cleaner data with our 96-well laminar washing system
With the Curiox Laminar Wash HT2000 System, you get all the benefits of gentle laminar cell washing in a format compatible with high-throughput and automated workflows.
Intuitive Touch-screen Interface
Quickly customize starting volume, flow rate and number of washes.
Automation-friendly Firmware & PCB
Easily integrate with automated robotic platforms
High-throughput 96-well Format
Process multiple samples at once with our gentle laminar washing technology.
Optional Buffer Exchange (BEX)
Use the touch-screen interface to choose from up to 8 different solutions.
What the HT2000 delivers:
Technology Overview
A quicker, simpler path to better data
With the HT2000’s laminar washing technology, cells are deposited onto a 96-well laminar wash plate, where a gentle flow of buffer washes away unwanted debris, such as unbound antibody or dye. Unlike with centrifugation, very little stress is placed upon the cells, ensuring the integrity of cell surface markers. The thoroughness of the laminar washing process produces cleaner, better segregated data than centrifugation-based wash methods and the gentleness of the technique enables high cell retention and viability.
“With the Curiox Laminar Wash™ we retain more cells with much less data variation between samples than our centrifuge process.”
Dr Jorgen Adolfsson, Linkoping University
Traditional Workflow
VS
Laminar Flow Technology
Compared to a traditional, centrifuge-driven cell washing workflow, laminar cell washing is simpler and faster. With the HT2000, you can get complete washing of 96 samples in just 3 minutes.
How our plate and nozzle design washes your cells
The power of Curiox’s laminar cell washing technology lies in the unique design of the 96-sample plate. Each plate contains an array of hydrophilic spots that suspension cells can rest on via gravity. The material surrounding each spot is highly hydrophobic, acting as an impenetrable wall that separates your samples.
Upon entry into the instrument, two nozzles descend onto each side of every spot, one to dispense wash buffer and the other to aspirate the buffer and debris away.
Each spot can wash as many as 10 million cells down to a single cell without the mechanical stress and cell losses associated with centrifugation.
See how dye is washed away from green fluorescent polystyrene beads during 2 minutes of 7 aspiration and dispense wash cycles. At the end of the cycle, the dye is gone but the polystyrene beads remain in the spot.
Scientific Data
Laminar washing delivers better cell retention and cleaner, clearer data
When performing cell dissociation in tumor bearing tissues, the tissue debris and enzymatic-induced fragments are primary factors affecting cell recovery for tumor infiltrating lymphocytes. For more invasive and immunologically challenging models, like B16F10-inoculated mice, recovery of limited numbers of TILs reduces chances for accurate characterization. The results shown here were demonstrated with an unchallenged mouse sample and the Laminar Wash technology. Laminar washing (with 1,000,000 tumor infiltrate cells) reveals increased viable cell count and reduced debris effects by laminar washing. The cleaner sample preparation increases lymphocyte recovery up to 90.6% as compared to centrifuge-harsh washing of 10.5%.
Laminar wash advances with neutrophils and monocytes retention for improved differential blood count
With 100ul of human whole blood, a comparison study using a 16-color flow cytometry panel focused on T cells was conducted by Laminar Washing. Whole blood lysed with ammonium chloride buffer was first centrifuged once to reduce volume, and then aliquoted to incubate with antibodies and washed on either Laminar Wash or centrifuge in polystyrene tubes. There is clear resolution of rare T cell populations such as MAIT, gamma-delta T cells and regulatory T cells with replacement of centrifuge with Laminar Wash in one wash step. The elevated retention for neutrophils and monocytes was observed and quantified via direct acquisition onto a BD Fortessa, allowing minimal cell perturbations.
Figure 2. Human red blood cells (RBCs) lysed with (NH4Cl) and washed using HT2000 Laminar Flow technology (top) show better cell retention and tighter cell populations than RBCs lysed and washed using centrifugation on samples in tubes (bottom), whether enumerated by CD45 staining or FSC-SSC scattering.
Featured Applications
The Laminar Wash system has been successfully demonstrated on various applications including immunophenotyping and CyTOF.
Flow Cytometry
Gently wash cells without centrifugal stress, allowing higher cell retention with less debris for acquisition and cell sorting.
Single Cell Sequencing
Wash cells without the stress of centrifugation that may impact gene/protein expression, cell count and viability.
Automated Workflows
Easily and affordably automate labor-intensive tasks, improve data quality and standardize with Laminar Wash technology.
COVID-19 Sample preparation
Generate reproducible data safely while reducing aerosolization of samples and variations between users by eliminating centrifugation.
Technical Specifications
Voltage Requirements |
100-240V |
Power Consumption |
24V/3.0A |
Dimensions |
310 mm H x 262mm W x 302 mm D |
Weight |
14kg (31lbs) |
Accessories and Components
Laminar Wash™ 96-well plate (with a regular lid) |
96-DC-CL-05 |
Laminar Wash™ 96-well plate (with a regular lid) *Customised |
96-DC-CL-05 |
HT Buffer Exchanger 5 |
DC-BX-01-05 |
HT Buffer Exchanger 10 |
DC-BX-01-10 |
Featured Resources
All you need to know about the laminar wash™ HT2000 System
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