Webinar On-Demand: Shift the Paradigm to Centrifuge-less Immuno-staining of Cells for Superior Viability, Recovery & Data

LAMINAR WASH™ MINI SYSTEM
Avoid the centrifuge and improve data quality and biosafety with the Laminar Wash MINI System.
The Laminar Wash MINI System employs the only suspension-cell sample preparation method that eliminates the centrifuge and the problems it introduces. It is an affordable bench-top instrument designed to produce the most quantitative and reproducible results for single cell sequencing, flow cytometry and mass cytometry.

What the MINI delivers:

  • Upgrade from the centrifuge — centrifuge-less, laminar flow based washing system
  • Improve cell viability and reduce stress on cells
  • Enhance cell retention
  • Reduce hands on time
TECHNOLOGY OVERVIEW
SEE THE LAMINAR WASH MINI IN ACTION

With the Laminar Wash technique, cells undergo a gentle wash without added stress, allowing a higher retention of cells for acquisition and cell sorting. In addition, cell surface markers are not modified and unbound antibodies are washed off efficiently, producing cleaner and better segregated data.

Each spot can process from a single cell to as many as 10 million cells without the mechanical stress and cell losses associated with centrifugation.

“I ran my first couple experiments on the Curiox Laminar Wash MINI last week and I am never going back to centrifugation! It is great!”
Senior Researcher, Leading Respiratory Teaching Hospital

TRADITIONAL

WORKFLOW

VS

LAMINAR FLOW

TECHNOLOGY

With the Laminar Wash technique, cells undergo a gentle wash without added stress, this enables >95% cell retention and results in superior data quality over microtiter plates/centrifugation
DATA USING THE LAMINAR WASH™ SYSTEM
Enhanced Cell Recovery post cell sorting for lower cell numbers

After freeze-thaw and cell sorting, a limited number of peripheral blood lymphocytes (less than 500,000) is resuspended into a sorting buffer prior to performing a CiteSeq™ staining and cell hashing. As compared to three cycles of centrifugation-based washing, the percentage of cell recovered and measured to be approximately 19% as compared to Laminar Wash’s cell recovery of more than 50% in 25ul leftover volume.  Data and image courtesy of ImmunAI.

Cell-hashing data reveals increased singlet and reduced doublet counts

Peripheral blood lymphocytes from humans were labelled with cell hashtags directly on the Laminar Wash 96 well plates and the derived sequence counts revealed less than 20% multiplets while the number of cells not stained with cell hashtag was reduced. The centrifuge-less Laminar Wash increases in singlet yield (up to 80%) which enhances more accurate identification for single cell targeting and helps higher sample multiplexing. Data and image courtesy of ImmunAI.

Flexible and optimized performance for using Cite-seq higher antibody panels

Laminar wash methodology yields higher cell retention and singlet distinction while enabling ease of wash protocol transfer to more complex proteogenomic reads (as seen here by up to 100 plus antibody panel). The increase in cell retention translates well to the amount of Cite-seq antibody to use and the data shows clear sample identification and clear antibody derived sequence-counts. Data and image courtesy of ImmunAI.

FEATURED APPLICATIONS
The Laminar Wash system has been successfully demonstrated on various applications including immunophenotyping and CyTOF.

FLOW CYTOMETRY

Improve your sample prep workflow and gently wash cells without stress, allowing higher cell retention and less debris for acquisition and cell sorting.
SINGLE CELL SEQUENCING
Wash cells without the stress of centrifugation that may impact gene/protein expression, cell count and viability.
TUMOR MICORENVIRONMENT
Recovery of TILs can be challenging from disocciated tumor samples. Enhance cell retention, improve reproducibility and reduce debris, background, artifacts and clogging.
COVID-19 SAMPLE PREPARATION
Improve biosafety by avoiding centrifugation and easily contain samples while reducing aerosolization, debris, background and clogging.
TECHNICAL SPECIFICATIONS

Voltage Requirements

100-240V

Power Consumption

24V/3.0A

Dimensions

232mm H x 275mm W x 187.5mm D
9.1in H x 10.8in W x 7.4in D

Weight

3.5kg (7.7 lbs)

ACCESSORIES AND COMPONENTS

Laminar Wash™ 16-well plate (with a regular lid)

96-DC-CL-05

Laminar Wash™ 16-well plate (with a regular lid) *Customised

96-DC-CL-05-CP

FEATURED RESOURCES
ALL YOU NEED TO KNOW ABOUT THE LAMINAR WASH™ HT2100 SYSTEM
A Novel Sample Preparation Method for Improved Tumor-Infiltrating Lymphocyte Recovery
Centrifuge-less Whole Blood Staining with Laminar Wash in a Biosafety Cabinet
Towards standardizing flow cytometry sample preparation protocols without centrifugation
RELATED PRODUCTS AND ACCESSORIES
MINI LAMINAR WASH STRIPS
Novel “wall-less” 16 strips for use with the Laminar Wash™ MINI System
LAMINAR WASH AUTO 1000
Complete Automation Solutions for Surface Staining and Intracellular Staining for Flow Cytometry

LAMINAR WASH HT2100

A reliable and easy-to-use benchtop instrument for high throughput flow cytometry labs.
Interested?
GET IN TOUCH TO FIND OUT HOW THE LAMINAR WASH SYSTEMS CAN IMPROVE YOUR WORKFLOW