Laminar Wash Plates

Eliminate centrifugation and the experimental variables it introduces.

Laminar Wash Plates

Innovative Nozzle Design

The Laminar Wash™ 96-well plate consists of an array of hydrophilic spots surrounded by a hydrophobic surface, which functions as a virtual wall that separates each spot.

What the Wash Plates deliver:
  • Upgrade from centrifugation using Laminar Wash plates
  • Enhance cell retention
  • Reduce hands-on time
  • Improve workflow
  • Improve cell viability and reduce stress on cells

See the Laminar Wash HT2000 in Action

Wells on the Laminar Wash plate have a hydrophilic surface inside low-profile walls, including two smaller, satellite areas, and are separated by a proprietary hydrophobic surface. These uniquely engineered plates maintain each sample as a separate droplet held in place by surface tension and hydrophobic interactions with the surrounding material.

LaminarWash_in_Action

Laminar Wash plates are used for cell processing of suspension cells for analytical assays. Each plate has 96 wells designed with two satellites to allow for dual-nozzle laminar flow processing via the HT2000 system. Each well has its own nozzles for quick and simultaneous washing of each well. Each well accomodates up to 70 ul (150 ul with an adaptor), at a maximum load of approximately 10 million cells.

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“Antonino Montalbano: We really have a nice recovery and the Curiox (Laminar Wash technology) has been great for samples that have a very low number of cells”

Dr. Antonino Montalbano, ImmunAI

Centrifuge-less cell processing for flow cytometry assays.

Traditional centrifuge-based preparation of cells are time consuming, labor intensive, waste expensive reagents, and stress cells. There is significant cell loss and incomplete washing resulting in problems with gating and cell segmentation.

Data using the Laminar Wash System

Enhanced Cell Recovery post cell sorting for lower cell numbers

Centrifuge-vs-Laminar-Wash-apl--new

After freeze-thaw and cell sorting, a limited number of peripheral blood lymphocytes (less than 500,000) is resuspended into a sorting buffer prior to performing a CiteSeq™ staining and cell hashing. As compared to three cycles of centrifugation-based washing, the percentage of cell recovered and measured to be approximately 19% as compared to Laminar Wash’s cell recovery of more than 50% in 25ul leftover volume.

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Laminar washing delivers better cell retention and cleaner, clearer data

When performing cell dissociation in tumor bearing tissues, the tissue debris and enzymatic-induced fragments are primary factors affecting cell recovery for tumor infiltrating lymphocytes. For more invasive and immunologically challenging models, like B16F10-inoculated mice, recovery of limited numbers of TILs reduces chances for accurate characterization. The results shown here were demonstrated with an unchallenged mouse sample and the Laminar Wash technology. Laminar washing (with 1,000,000 tumor infiltrate cells) reveals increased viable cell count and reduced debris effects by laminar washing. The cleaner sample preparation increases lymphocyte recovery up to 90.6% as compared to centrifuge-harsh washing of 10.5%.

TILs B16F10
Ordering Information
Laminar Wash™ 16-well plate (with a regular lid) 16-DC-CL-10
Laminar Wash™ 16-well plate (with a regular lid) *Customised 16-DC-CL-10-CP
Laminar Wash™ 96-well plate (with a regular lid) 96-DC-CL-05
Laminar Wash™ 96-well plate (with a regular lid) *Customised 96-DC-CL-05-CP
FEATURED RESOURCES

All you need to know about the Laminar Wash HT2000 System

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