Laminar Wash™ Plates
Eliminate centrifugation and the experimental variables it introduces.
Innovative Nozzle Design
The Laminar Wash™ 96-well plate consists of an array of hydrophilic spots surrounded by a hydrophobic surface, which functions as a virtual wall that separates each spot.
What the Wash Plates deliver:
Technology Overview
SEE THE LAMINAR WASH HT2000 IN ACTION
Wells on the Laminar Wash plate have a hydrophilic surface inside low-profile walls, including two smaller, satellite areas, and are separated by a proprietary hydrophobic surface. These uniquely engineered plates maintain each sample as a separate droplet held in place by surface tension and hydrophobic interactions with the surrounding material.
Laminar Wash plates are used for cell processing of suspension cells for analytical assays. Each plate has 96 wells designed with two satellites to allow for dual-nozzle laminar flow processing via the HT2000 system. Each well has its own nozzles for quick and simultaneous washing of each well. Each well accomodates up to 70 ul (150 ul with an adaptor), at a maximum load of approximately 10 million cells.
“We really have a nice recovery and the Curiox (Laminar Wash technology) has been great for samples that have a very low number of cells”
Dr. Antonino Montalbano, ImmunAI
CENTRIFUGE-LESS CELL PROCESSING FOR FLOW CYTOMETRY ASSAYS
Traditional centrifuge-based preparation of cells are time consuming, labor intensive, waste expensive reagents, and stress cells. There is significant cell loss and incomplete washing resulting in problems with gating and cell segmentation.
How Laminar Wash™ Works
Enhanced Cell Recovery post cell sorting for lower cell numbers
The power of Curiox’s laminar cell washing technology lies in the unique design of the 96-sample plate. Each plate contains an array of hydrophilic spots that suspension cells can rest on via gravity. The material surrounding each spot is highly hydrophobic, acting as an impenetrable wall that separates your samples.
Upon entry into the instrument, two nozzles descend onto each side of every spot, one to dispense wash buffer and the other to aspirate the buffer and debris away.
Each spot can wash as many as 10 million cells down to a single cell without the mechanical stress and cell losses associated with centrifugation.
Scientific Data
Laminar washing delivers better cell retention and cleaner, clearer data
When performing cell dissociation in tumor bearing tissues, the tissue debris and enzymatic-induced fragments are primary factors affecting cell recovery for tumor infiltrating lymphocytes. For more invasive and immunologically challenging models, like B16F10-inoculated mice, recovery of limited numbers of TILs reduces chances for accurate characterization. The results shown here were demonstrated with an unchallenged mouse sample and the Laminar Wash technology. Laminar washing (with 1,000,000 tumor infiltrate cells) reveals increased viable cell count and reduced debris effects by laminar washing. The cleaner sample preparation increases lymphocyte recovery up to 90.6% as compared to centrifuge-harsh washing of 10.5%.
Ordering Information
Laminar Wash™ 16-well plate (with a regular lid) |
16-DC-CL-10 |
Laminar Wash™ 16-well plate (with a regular lid) *Customised |
16-DC-CL-10-CP |
Laminar Wash™ 96-well plate (with a regular lid) |
96-DC-CL-05 |
Laminar Wash™ 96-well plate (with a regular lid) *Customised |
96-DC-CL-05-CP |
Featured Resources
All you need to know about the laminar wash™ HT2000 System
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