Laminar Wash Plates
Eliminate centrifugation and the experimental variables it introduces.
Innovative Nozzle Design
The Laminar Wash™ 96-well plate consists of an array of hydrophilic spots surrounded by a hydrophobic surface, which functions as a virtual wall that separates each spot.
What the Wash Plates deliver:
- Upgrade from centrifugation using Laminar Wash plates
- Enhance cell retention
- Reduce hands-on time
- Improve workflow
- Improve cell viability and reduce stress on cells
Data using the Laminar Wash System
Enhanced Cell Recovery post cell sorting for lower cell numbers
After freeze-thaw and cell sorting, a limited number of peripheral blood lymphocytes (less than 500,000) is resuspended into a sorting buffer prior to performing a CiteSeq™ staining and cell hashing. As compared to three cycles of centrifugation-based washing, the percentage of cell recovered and measured to be approximately 19% as compared to Laminar Wash’s cell recovery of more than 50% in 25ul leftover volume.
Laminar washing delivers better cell retention and cleaner, clearer data
When performing cell dissociation in tumor bearing tissues, the tissue debris and enzymatic-induced fragments are primary factors affecting cell recovery for tumor infiltrating lymphocytes. For more invasive and immunologically challenging models, like B16F10-inoculated mice, recovery of limited numbers of TILs reduces chances for accurate characterization. The results shown here were demonstrated with an unchallenged mouse sample and the Laminar Wash technology. Laminar washing (with 1,000,000 tumor infiltrate cells) reveals increased viable cell count and reduced debris effects by laminar washing. The cleaner sample preparation increases lymphocyte recovery up to 90.6% as compared to centrifuge-harsh washing of 10.5%.