Webinar On-Demand: Shift the Paradigm to Centrifuge-less Immuno-staining of Cells for Superior Viability, Recovery & Data

MULTIPLEX IMMUNOASSAYS
Save sample and reduce costs by miniaturizing your multiplex immunoassays.

Technology

DropArray Technology allows you to easily miniaturize your multiplex assays.

Benefits

Increase the number of samples you can run from a single kit.

Scientific Data

See published data from miniaturized multiplex cytokine assays.

Resources

Useful links for multiplex immunoassays.

Overview

CHANGE THE WAY YOU PROCESS CELLS TO GET RELIABLY CONSISTENT DATA

Reliable Data from Miniaturization

It is known that bead-based assays sometimes suffer from bead aggregation with plasma, blood, and other challenging biological samples which affects detectability of proteins and jeopardizes quality of data. Scientific studies have shown that DropArray may reduce bead aggregation and may better permit reading beads individually reporting the signals of target proteins as they are.

“…the DA-Bead method for analysis was comparable to, if not superior to, the conventional method in terms of consistency/precision, accuracy, sensitivity and dynamic range and these results are not specific to sample type, reagents, or commercial vendor.”
Leroy Versteeg, Baylor College of Medicine

THE CURIOX
DA-BEAD PLATE

VS

TRADITIONAL PLATE
TECHNOLOGY

A) Incubation method principle in traditional plate (left) and DA-Bead Plate (right). Beads are freely floating with shaking in traditional well at 75µl volume vs. beads are fixed in a 15 µl drop in DA-Bead Plate by a magnet below the well and shaken.

(B) Washing method principle in traditional plate (left) and DA-Bead Plate (right). Magnet array with traditional plate either super packs beads at the side of a traditional well or keep beads as a monolayer resulting in aspiration of beads and/or incomplete washing with the nozzle/pipet based dispenser. Conversely, DA-Bead Plate magnet configuration maintains beads as a monolayer (right) resulting in complete washing with gentle laminar flow and minimal residual wash buffer leftover.

Benefits
MULTIPLEX IMMUNOASSAYS

Multiplex bead-based assays are robust and generate highly sensitive data from a variety of samples types. However, these assays are costly and involve expensive reagents and often valuable samples. Curiox’s new DropArray™ plates allow users to analyze the same number of analytes at a fraction of the cost and sample consumption. DropArray™ plates use 5-fold smaller volumes, so the same amount of reagent can produce 5x more data points.

  • A 5-fold miniaturization reduces total assay costs by 60–80%
  • DropArray™ plates allow 5x more wells to be run from a single assay kit
Featured Resource
BEAD-BASED CYTOKINE ASSAY ON WALL LESS PLATES: DIRECT COMPARISON TO CONVENTIONAL PLATE ASSAY
Scientific Data
DATA USING THE LAMINAR WASH SYSTEMS
Peritoneal Fluid Cytokine Multiplex Analysis

Peritoneal fluid cytokines show distinct delineation of endometriosis subphenotypes. Partial least squares regression (PLSR) models separated (A) ovarian endometriomas from peritoneal endometriosis, (B) ovarian endometriomas from deep infiltrating endometriosis, and (C) peritoneal endometriosis from deep infiltrating endometriosis. The principal component (PC) scores show good separation of endometriosis subphenotypes by using PF cytokines. (D–F). Corresponding PLSR coefficient analyses reveal cytokine signatures delineating the various subphenotypes. Elevated cytokines associated with a particular endometriosis subphenotype (OE, PE, or DIE) relative to its comparator appear in the same upper or lower half of the plot.

https://doi.org/10.3390/ijms21103515

Multiplex Immunoassay for a Cytokine Re-stimulation Assay

Cytokine profiles of BALB/c mice immunized with rAs37 formulated with Alhydrogel, MPL, and AddaVax. Cytokine levels were determined in supernatants of splenocytes after being re-stimulated with rAs37 (25 μg/ml) for 48 hours. Cytokine values measured in unstimulated samples were subtracted from the cytokine values from the corresponding rAs37 re-stimulated samples. Data are presented as means and individual values for each group (n = 5). *p< 0.05, **p< 0.01.

https://doi.org/10.1371/journal.pntd…

FEATURED RESOURCES
ALL YOU NEED TO KNOW ABOUT THE DROPARRAY SYTEMS
Bead-based Immunoassays with DA-Bead Plate: Milliplex Assays
DropArray™, a Wall‐Less 96‐Well Plate for Uptake and Immunofluorescence Microscopy, Confirms CD22 Recycles
Transferring Luminex® cytokine assays to a wall-less plate technology: Validation and comparison study with plasma and cell culture supernatants.
RECENT PUBLICATIONS & RESOURCES

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RELATED PRODUCTS AND ACCESSORIES
LAMINAR WASH HT2000
Reliable Multiplex Immunoassays Data from Miniaturization
LAMINAR WASH AUTO 1000
Wall-less plate technology for miniaturizing bead-based immunoassays enabling small sample volumes
LAMINAR MINI SYSTEM
An affordable, benchtop instrument for low-throughput sample preparation for flow cytometry
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