Curiox Resources for Laminar Wash
This is where you can learn about various applications that benefit from Laminar Wash technology and links to useful resources.
EFFORTS TO STANDARDIZE FLOW CYTOMETRY
EuroFlow consists of 20 academic research centers and one industry group that have been identified as experts in the fields of both flow cytometry and molecular diagnostics. Their aim is to standardize and create protocols for those that seek to use quantitative flow cytometry for diagnostic applications. They have a number of already published protocols on their website and useful references.
NIST (National Institute of Standard and Technology)
This United States government organization has a program on quantitative flow cytometry measurements with an objective of developing reference materials, methodology, and procedure to enable quantitative measurements on cells. They have developed several reference standards and a reference protocol is in development using CD4 expression on healthy human T lymphocytes as a reference biomarker control.
Four Key Publications on Quantitative Flow Cytometry
There is a trend in cell therapy and drug development to make flow cytometry assays more quantitative. Here are four key publications that show examples of quantitative flow cytometry assays that can help you develop better assays in your work.
Cell Therapy/Cell Manufacturing
Quadrini KJ, Hegelund AC, Cortes KE, et al. Validation of a flow cytometry-based assay to assess C5aR receptor occupancy on neutrophils and monocytes for use in drug development. Cytometry Part B: Clinical Cytometry. 2016;90(2):177-190.
Singh H, Figliola MJ, Dawson MJ, et al. Manufacture of Clinical-Grade CD19-Specific T Cells Stably Expressing Chimeric Antigen Receptor Using Sleeping Beauty System and Artificial Antigen Presenting Cells. PLOS ONE. 2013;8(5):e64138.