Professor Mats Bemark, Ph.D.‘s lab at the University of Gothenburg in Sweden studies how the immune system responds to mucosal infections, including SARS-CoV-2 and cholera. His research focuses on interrogating these interactions using advanced analytical methods, including flow cytometry and single-cell transcriptomics (scRNA-seq). A key to unlocking the potential of these studies is addressing sample prep challenges, particularly when starting cell numbers are limited.

Curiox Biosystems Marketing & Content Manager Geoffrey Feld, Ph.D. got things started with an introduction to Laminar Wash technology and its common benefits for improving single-cell sequencing sample prep.

Dr. Bemark's talk focused on:

  • The basics of antigen-specific lymphocyte biology
  • The theory and benefits of multiplexing single-cell transcriptomics with cell hashing and proteogenomic antibodies
  • The challenges researchers face with a limited number of cells in a given sample
  • Sample prep strategies for collecting and enriching rare immune cells for single-cell analysis
  • Why proper cell washing is integral to study success
  • How Laminar Wash enabled such studies in a mouse model infection of cholera and severe COVID-19 hospitalization in humans

The two speakers also answered several excellent questions from the attendees for the final few minutes.