Safe and Consistent Sample Prep in COVID-19 Research & Vaccine Development

Sample prep is difficult, and is a source of inconsistency between users and samples. Using a centrifuge is also a multi-step process that can be easily performed differently by different users. Finally, centrifugation of samples can lead to aerosolization, which should be avoided.

Contain Infectious Samples in a Biosafety Cabinet from Start to Finish

The World Health Organization Laboratory guidance for research on the novel coronavirus SARS-CoV2 is that “all technical procedures should be performed in a way that minimizes the generation of aerosols and droplets.”
Table 1. Risk prioritization of selected routine laboratory tasks
Task or activity Exposure risk
Potential hazard Likelihood Consequence Risk rating
Subculturing blood culture bottle Needle stick — percutaneous inoculation Likely Infection; medical treatment High
Aerosols — inhalation Moderate Infection; medical treatment Medium
Splash — direct contact with mucous membranes Moderate Infection; medical treatment High
Centrifugation Aerosols — inhalation Likely Infection; medical treatment High
Performing Gram stain Aerosols from flaming slides Moderate Colonization; infection Moderate
Preparing AFB smear only Aerosols from sputum or slide preparation Likely Illness; medical treatment; disease High
Performing catalase testing Aerosols — mucous membrane exposure Unlikely Colonization; infection Low
AFB culture work-up Aerosols — inhalation Likely Illness; medical treatment; disease High
Abbreviation: AFB = acid-fast bacillus.

From the CDC Guidelines for Safe Work Practices in Human and Animal Medical Diagnostic Laboratories Recommendations of a CDC-convened, Biosafety Blue Ribbon Panel (Source)

New Method to Prepare Immune Cell Populations in COVID-19 Samples

A new configuration of the Laminar Wash™ HT1000 System packaged with STEMCELL Technologies’ EasySep™ RBC Depletion Reagent.
  • Containment of blood sample processing within a biosafety cabinet
  • Washing without centrifugation, eliminating aerosolization of potentially infectious samples
  • Superior data analysis because cells are not stressed by lysis or centrifugation and show improved staining index
STEMCELL Technologies’ EasySep™ RBC Depletion Reagent and the Laminar Wash™ HT1000 System.
STEMCELL Technologies’ EasySep™ RBC Depletion Reagent and the Laminar Wash™ HT1000 System.

How does Curiox technology help our Vaccine customers?

“The Laminar Washer is safer than flicking (minimal exposure to infectious materials) and requires less space than centrifuges. Our samples are fragile and prone to cell death but the LW  promotes cell viability and stability and has proven consistency at low cell counts producing reliable and reproducible results and also prevents cross-contamination of samples. It saves time, easy use and fewer lab techs are needed and has proven operator consistency Inter & Intra-Plate. Requires minimal maintenance and is also cost effective.”
Blanca Ponce-Ngo, Montefiore Medical Center

Magnetic depletion of erythrocytes with LW gave clear resolution of cell populations

  1. Clear segregation of lymphocyte, monocyte and neutrophil populations with low debris scattering.
  2. Left shift of granulocyte suggests improved condition

Laminar Wash technology does help our Vaccine customers!

Retain

Biosafety: No centrifugal aerosols and enable safety hood installation.
Control
: Eliminate plate flicking and cross contamination.
Sample quality
: Cell viability, cell numbers and cell separation.

Reduce

Cell stress:  Sample debris, cell clumping and cytometer clogging.
Data variation: And operator inconsistency.
Workflow steps: And time to results.

Repeat

And optimise: For multiple sample types, protocols and applications
And integrate
: With laboratory automation.