Safe and Consistent Sample Prep in COVID-19 Research & Vaccine Development
Sample prep is difficult, and is a source of inconsistency between users and samples. Using a centrifuge is also a multi-step process that can be easily performed differently by different users. Finally, centrifugation of samples can lead to aerosolization, which should be avoided.
Contain Infectious Samples in a Biosafety Cabinet from Start to Finish
|Table 1. Risk prioritization of selected routine laboratory tasks|
|Task or activity||Exposure risk|
|Potential hazard||Likelihood||Consequence||Risk rating|
|Subculturing blood culture bottle||Needle stick — percutaneous inoculation||Likely||Infection; medical treatment||High|
|Aerosols — inhalation||Moderate||Infection; medical treatment||Medium|
|Splash — direct contact with mucous membranes||Moderate||Infection; medical treatment||High|
|Centrifugation||Aerosols — inhalation||Likely||Infection; medical treatment||High|
|Performing Gram stain||Aerosols from flaming slides||Moderate||Colonization; infection||Moderate|
|Preparing AFB smear only||Aerosols from sputum or slide preparation||Likely||Illness; medical treatment; disease||High|
|Performing catalase testing||Aerosols — mucous membrane exposure||Unlikely||Colonization; infection||Low|
|AFB culture work-up||Aerosols — inhalation||Likely||Illness; medical treatment; disease||High|
|Abbreviation: AFB = acid-fast bacillus.|
New Method to Prepare Immune Cell Populations in COVID-19 Samples
- Containment of blood sample processing within a biosafety cabinet
- Washing without centrifugation, eliminating aerosolization of potentially infectious samples
- Superior data analysis because cells are not stressed by lysis or centrifugation and show improved staining index
How does Curiox technology help our Vaccine customers?
Magnetic depletion of erythrocytes with LW gave clear resolution of cell populations
- Clear segregation of lymphocyte, monocyte and neutrophil populations with low debris scattering.
- Left shift of granulocyte suggests improved condition
Laminar Wash technology does help our Vaccine customers!
Biosafety: No centrifugal aerosols and enable safety hood installation.
Control: Eliminate plate flicking and cross contamination.
Sample quality: Cell viability, cell numbers and cell separation.
Cell stress: Sample debris, cell clumping and cytometer clogging.
Data variation: And operator inconsistency.
Workflow steps: And time to results.
And optimise: For multiple sample types, protocols and applications
And integrate: With laboratory automation.