Webinar On-Demand: Shift the Paradigm to Centrifuge-less Immuno-staining of Cells for Superior Viability, Recovery & Data

SINGLE-CELL SEQUENCING
Treat your cells gently in preparation for critical single-cell genomic and proteogenomic applications.

Technology

Laminar Wash technology gently washes cells, minimizing gene expression changes during sample prep.

Benefits

Improve cell viability and reduce stress on cells; cleaner samples with fewer doublets and debris.

Scientific Data

See examples of enhanced cell retention and reduced doublets.

Resources

Links to resources and publications.

Overview
CHANGE THE WAY YOU PROCESS CELLS TO GET RELIABLY CONSISTENT DATA

Single-cell genomics and proteogenomics are rapidly growing. Nature Methods named multimodal omics the Method of the Year for 2019. As more scientists use single-cell techniques, they find that sample prep is paramount to producing high-quality data.

Cell clumping, poor retention of cells with already low cell counts, and unwashed residual nucleic acids are just a few symptoms of poor cell washing that impacts single-cell sequencing and proteogenomics. Centrifugation also induces cellular stress on samples, resulting in erroneous gene expression results.

Sample prep is extremely important in single-cell genomics and proteogenomics, especially for single-cell gene expression and immune profiling. While mRNA transcripts are being barcoded, cellular mRNA leakage (increased background) or mRNA degradation (decreased signal) significantly impacts these sensitive measurements. Unlike with flow cytometry, gating out debris or groups of cells is not possible in single-cell sequencing. The cell preparation must be as clean as possible and yield single cells.

10X Genomics’ Sample Preparation Demonstrated Protocol Fresh Frozen Human Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing (Part Number CG00039 Rev C) uses centrifugation to wash cells and warns, “However, be careful not to over-centrifuge as increased centrifugation speed can compromise the cell integrity and viability.” A study published in Nature Methods shows that single cell isolation procedures induce transcriptome-wide changes in cells. So, it makes sense that if there is a gentler method to wash cells scientists should use it.

Using the Laminar Wash system saves time with fewer manual manipulations. Gently washing cells without unnatural centrifugal forces maintains cell integrity and viability, while ensuring gene or protein expression changes are biological, instead of due to workflow.

Laminar Wash technology has been added as part of the official TotalSeq™ Protocol. It simplifies the processing of samples with low cell counts, enabling high-throughput.

“Curiox (Laminar Wash) really helps retain high numbers of cells”
Dr. Antonino Montalbano, ImmunAI

TRADITIONAL

WORKFLOW

VS

LAMINAR FLOW

TECHNOLOGY

With the Laminar Wash technique, cells undergo a gentle wash without added stress, this enables >95% cell retention and results in superior data quality over microtiter plates/centrifugation
Benefits
LAMINAR WASH IS AN AFFORDABLE AND SIMPLE SOLUTION TO AUTOMATE SAMPLE PREP FOR FLOW CYTOMETRY:
  • Improved resolution of cell populations, including rare cell types
  • Reduced debris and cellular aggregation
  • Significant time saving of 70~90 % in processing cells, allowing scientists to spend more time on analysis and science
  • Keeping cells un-stressed in their native state, improving viability
  • Practical integration with cell analysis workflows, particularly in combination with automated data analysis
Featured Resource
Single-Cell Transcriptomic Analyses Define Distinct Peripheral B Cell Subsets and Discrete Development Pathways. Stewart, et al. (2021) Front. Immunol.
Scientific Data
DATA USING THE LAMINAR WASH™ SYSTEMS
Improved retention, better multiplexing from single cell proteogenomic experiments.

Figure 1: Laminar Wash generates better cell hashing results

Samples processed with a Curiox Laminar Wash system yield fewer doublets, fewer negative results and higher numbers of singlets. Data courtesy of ImmunAI, adapted from Dr. Antonio Montalbano’s webinar with Curiox Biosystems.

Improved Operator Consistency by Automating Only the Wash Step

Figure 2: Laminar Wash Retains Higher Numbers of Cells.

Laminar Wash consistently retained higher numbers of cells, compared to traditional centrifuge-based methods. This is especially true for samples that have a very low number of cells. Data courtesy of ImmunAI, adapted from Dr. Antonio Montalbano’s webinar with Curiox Biosystems.

FEATURED RESOURCES
ALL YOU NEED TO KNOW ABOUT THE LAMINAR WASH™ HT2100 SYSTEM
Walk-Away Automation of Cell Immunostaining Assays by Centrifuge-Less Laminar Wash™
Centrifuge-less Whole Blood Staining with Laminar Wash in a Biosafety Cabinet
Enhancing Cell Recovery and Debris Removal for Frozen Lymphocytes for Single Cell Multi-Omic
RELATED PRODUCTS AND ACCESSORIES
LAMINAR WASH HT2000
A reliable and easy-to-use benchtop instrument for high throughput flow cytometry labs.
LAMINAR WASH AUTO 1000
Accelerate high-throughput flow and mass cytometry workflows with fully automated sample preparation.
LAMINAR MINI SYSTEM
An affordable, benchtop instrument for low-throughput sample preparation for flow cytometry.
Get Started
AUTOMATE YOUR FLOW CYTOMETRY WORKFLOW