Surfaceome mapping of primary human heart cells with CellSurfer uncovers cardiomyocyte surface protein LSMEM2 and proteome dynamics in failing hearts

Cell surface proteins (surfaceome) play a significant role in the development of novel target therapies. Therefore, a comprehensive surfaceome profiling approach is necessary to identify and quantify specific markers for effective therapeutic targeting. The challenge most developers face is the lack of data due to complex methodologies, rare cell types and/or small number of cells. 

This study conducted by the exceptional scientists at the CardiOmics Program, Center for Heart and Vascular Research and Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, as it demonstrates overcoming these challenges by incorporating the novel and gentle sample preparation method called the Curiox Laminar Wash Technology, a centrifuge-less system designed to enable staining and washing of suspension cells at a fraction of the hands-on time while yielding higher cell retention and viability. As well as a new analytical platform called the CellSurfer, which enables quantitative surfaceome profiling regardless of sample sizes resulting in the detection of cardiomyocyte surface protein LSMEM2 and a comprehensive surfaceome repertoire. 

Download this article to learn more about: 

Identification of cell surface protein (surfaceome) LSMEM2 as a potential cardiomyocyte surface marker  

Quantitative surfaceome profiling with the analytical platform, CellSurfer and its advantages over other methods such as transcriptomics, computational approaches and generic proteomics 

Enhanced sample preparation application with the Curiox Laminar Wash Technology despite limited quantity of cells or complexity of the sample.