Protocol to characterize immune cell subpopulations in cerebrospinal fluid of patients with neuroinflammatory diseases using mass cytometry
Gallaccio, G., Wang, M., Schlickeiser, S., Kunkel, D., Böttcher, C., & Fernández-Zapata, C. (2024). STAR Protocols, 5(2), 103038. https://doi.org/10.1016/j.xpro.2024.103038 Phenotypic and compositional changes of immune cells in cerebrospinal fluid (CSF) can be used as biomarkers to help diagnose and track disease activity for neuroinflammatory and neurodegenerative diseases. Here, we present a workflow to perform high-dimensional […]
A novel mass cytometry protocol optimized for immunophenotyping of low-frequency antigen- specific T cells
Balz, K., Grange, M., Pegel, U., Karamya, Z. A., Mello, M., Zhou, X., Berger, T., Bloch, K., Dunham, D., Chinthrajah, S., Nadeau, K., Luche, H., & Skevaki, C. (2024b). Frontiers in Cellular and Infection Microbiology, 13. https://doi.org/10.3389/fcimb.2023.1336489 Understanding antigen-specific T-cell responses, for example, following virus infections or allergen exposure, is of high relevance for the […]
Surfaceome mapping of primary human heart cells with CellSurfer uncovers cardiomyocyte surface protein LSMEM2 and proteome dynamics in failing hearts
READ NATURE Cell surface proteins (surfaceome) play a significant role in the development of novel target therapies. Therefore, a comprehensive surfaceome profiling approach is necessary to identify and quantify specific markers for effective therapeutic targeting. The challenge most developers face is the lack of data due to complex methodologies, rare cell types and/or small number […]
Benefits of Using the Curiox Laminar Wash System in Mass Cytometry Experiments
Now Watchinghttps://www.youtube.com/watch?v=qOP26iVEnQs Description Jorgen Adolfsson is head of the Mass Cytometry Unit at Linköping University in Sweden, which is part of the SciLifeLab National Infrastructure. In this webinar Jorgen speaks about his experience using the Laminar Wash system. Jorgen describes reduction of antibody usage by ~50% with preserved signal and reproducibility. Laminar Wash technology has […]