Profiling Circulating T cells and Tumor Landscape for Predicting Responses to PD-1 blockade in Patients with Non-Small Cell Lung Cancer
Dutta, N. (2024, September 5). https://gupea.ub.gu.se/handle/2077/81804 Immune checkpoint blockade (ICB) has significantly improved outcomes in non-small cell lung cancer (NSCLC), yet only a subset of patients achieves durable responses. This underscores the need for effective methods to assess ICB efficacy. This thesis investigates the response to PD-1 blockade in NSCLC patients across stages I to […]
A unified model-based framework for doublet or multiplet detection in single-cell multiomics data
Hu, H., Wang, X., Feng, S., Xu, Z., Liu, J., Heidrich-O’Hare, E., Chen, Y., Yue, M., Zeng, L., Rong, Z., Chen, T., Billiar, T., Ding, Y., Huang, H., Duerr, R. H., & Chen, W. (2024). Nature Communications, 15(1). https://doi.org/10.1038/s41467-024-49448-x Droplet-based single-cell sequencing techniques rely on the fundamentalassumption that each droplet encapsulates a single cell, enabling […]
Protocol to characterize immune cell subpopulations in cerebrospinal fluid of patients with neuroinflammatory diseases using mass cytometry
Gallaccio, G., Wang, M., Schlickeiser, S., Kunkel, D., Böttcher, C., & Fernández-Zapata, C. (2024). STAR Protocols, 5(2), 103038. https://doi.org/10.1016/j.xpro.2024.103038 Phenotypic and compositional changes of immune cells in cerebrospinal fluid (CSF) can be used as biomarkers to help diagnose and track disease activity for neuroinflammatory and neurodegenerative diseases. Here, we present a workflow to perform high-dimensional […]
Longitudinal single-cell analysis of SARS-CoV-2–reactive B cells uncovers persistence of early-formed, antigen-specific clones
Scharf, L., Axelsson, H., Emmanouilidi, A., Mathew, N. R., Sheward, D. J., Leach, S., Isakson, P., Smirnov, I. V., Marklund, E., Miron, N., Andersson, L., Gisslén, M., Murrell, B., Lundgren, A., Bemark, M., & Angeletti, D. (2022). JCI Insight, 8(1). https://doi.org/10.1172/jci.insight.165299 Understanding persistence and evolution of B cell clones after COVID-19 infection and vaccination is […]
Using high throughput technologies to understand heterogeneous drug response
GE Boyle (2023), University of Washington Heterogeneity is an inherent quality of biological systems, and is one of the properties that facilitates adaptation of organisms to new environments and stimuli. However, the scientific method relies on probabilistic reasoning wherein sample measurements are assumed to represent the population as a whole. In situations where this assumption […]
Laminar Wash MINI000 Virtual Demonstration with CITE-Seq Protocol
Now Watchinghttps://www.youtube.com/watch?v=HrvllFVj3K0 This video demonstrates the daily operation and utility of the Laminar WashTM MINI1000 system, hosted by Curiox Biosystems scientist Amira Amilia, and features a common protocol example in setting up a CITE-Seq (Cellular Indexing of Transcriptomes and Epitopes by Sequencing) staining assay. Comprised of a proprietary 16-well “wall-less” strip and a fluidics instrument, the MINI1000 is the […]
Brochure—Mini1000 System
Download MINI 1000 Brochure
Single Cell Genomics Workflow with Laminar Wash (LW) in Biosafety Cabinet
Now Watchinghttps://www.youtube.com/watch?v=3bMAZ9cP75g Description Single cell genomics provide valuable information on the profiles and differences between individual cells from a heterogenous cell population, such as immune profiling of tumor microenvironments. Preparation of single cell suspensions for single cell genomic applications is crucial to obtaining high quality sequencing data. Factors such as poor cell retention and contamination […]
Laminar Wash MINI1000 Setup and Workflow in Biosafety Cabinet
Now Watchinghttps://www.youtube.com/watch?v=wbZIFLzIwEc Description This video offers a detailed guide to using and maintaining the Laminar Wash MINI 1000 System for flow cytometry sample preparation. As demonstrated by our scientist in the video, setting up of the Laminar Wash MINI 1000 is straightforward, including pre-programmed priming steps. The Laminar Wash MINI 1000 System can be easily […]
User Manual—MINI1000 System
Laminar Wash MINI1000 User Manual