Protocol to characterize immune cell subpopulations in cerebrospinal fluid of patients with neuroinflammatory diseases using mass cytometry

Gallaccio, G., Wang, M., Schlickeiser, S., Kunkel, D., Böttcher, C., & Fernández-Zapata, C. (2024). STAR Protocols, 5(2), 103038. https://doi.org/10.1016/j.xpro.2024.103038 Phenotypic and compositional changes of immune cells in cerebrospinal fluid (CSF) can be used as biomarkers to help diagnose and track disease activity for neuroinflammatory and neurodegenerative diseases. Here, we present a workflow to perform high-dimensional […]

An immunophenotype-coupled transcriptomic atlas of human hematopoietic progenitors

Zhang, X., Song, B., Carlino, M. J., Li, G., Ferchen, K., Chen, M., Thompson, E. N., Kain, B. N., Schnell, D., Thakkar, K., Kouril, M., Jin, K., Hay, S. B., Sen, S., Bernardicius, D., Ma, S., Bennett, S. N., Croteau, J., Salvatori, O., . . . Grimes, H. L. (2024). Nature Immunology, 25(4), 703–715. https://doi.org/10.1038/s41590-024-01782-4 […]

Future-Proofing Single Cell Applications

Now Watchinghttps://youtu.be/wL2tsRsL9TA Technology has evolved from those early days of batching thousands of cells together to decrypt the millions of base pairs that make up genetic information. Cellular Indexing of Transcriptomes and Epitopes by sequencing (CITE-seq), brings together surface protein phenotyping (similar to flow cytometry) and single-cell RNA sequencing (scRNA-seq). This is achieved by using […]

Overcoming challenges of Nuclei and Cell Preparation: A scientific perspective by S2 genomics and Curiox Story

Now Watchinghttps://youtu.be/inLymDAn4EI Single-cell transcriptome analysis has been extensively applied in humans and animal models to uncover gene expression heterogeneity between the different cell types of a tissue or an organ. Upstream processing may introduce inherent challenges that mask true analysis and increase user variability. Here we present a simple, yet combinatorial strategy from buffer and […]

Impact of Novel Curiox Laminar Wash on Nuclei Retention and Other Downstream Applications

Now Watchinghttps://youtu.be/U0oWMffKFlM Cell type-specific nuclei populations can be used for a wide range of downstream sequencing applications including transcriptomic and epigenomic analyses. Cerevance’s breakthrough use of Laminar Wash™ Technology in their proprietary NETSseq platform has enabled an improvement in nuclei retention as well as reagent cost reductions and time savings. This Labroots Webinar features David […]

Comprehensive Benchmarking of CITE-seq versus DOGMA-seq Single Cell Multimodal Omics

READ BIORXIV While single-cell multiomics techniques represent a growing dictionary for generating enormous insight into cell biology, objective assessments of utility, strengths, and weaknesses are lacking. Researchers at the the University of Pittsburgh compared trimodal (transcription, epitopes, and chromatin accessibility) DOGMA-Seq to bimodal (transcription and epitopes) CITE-seq to benchmark and optimize sample prep using Laminar […]

Enabling Single-cell Multiomics and Cell Hashing in Studies with Limited Numbers of Cells

Now Watchinghttps://youtu.be/LagyuoWvELo Our October 19, 2021 webinar featured University of Gothenburg Associate Professor of Immunology Mats Bemark, Ph.D. and Curiox Biosystems Marketing & Content Manager, Geoffrey Feld, Ph.D. Dr. Bemark studies mucosal immunology, with particular focus on the gut and gut-related infections like cholera, and recently has been working on COVID-19 with human severe disease samples. His […]

Nuclei Need Nurturing, Too

Laminar Wash™ benefits of automation and gentle cell-based sample preparation can also be extended to nuclei Analytical methods on individual cells provide an enormous amount of useful information. But not all tissue types lend themselves to preparing single cell suspensions necessary for flow cytometry or single-cell ‘omics analysis. Homogenization and dissociation of interconnected tissues such […]