How does the difference in reagent volume used with the DA Bead Plate impact CVs?

CVs are a result of variability and this variability can come from the user or the assay. In general, dispensing smaller volumes has more error than dispensing larger volumes, but this is very user/technique-dependent and pipette quality driven. It is known that the higher intra-assay CVs for bead-based assays stem from a combination of steps, mostly from dispensing, washing, and Ab-Ag binding on the bead surface. The DropArray system may produce higher CVs purely due to dispensing in smaller volumes. However, uniform and consistent specific binding with minimal non-specific binding and better washing with minimal loss of beads has been shown in studies to reduce CVs. The overall impact of the lower volume will depend on the specific test conducted and can be analyzed using comparative studies.