Improve your vaccine studies using techniques such as immunophenotyping and single cell genomics by using Laminar Wash™ technology.
Laminar Wash technology keeps samples in their native state by treating them gently.
Reduce aerosolization and enhance cell retention in your
See examples of enhanced cell retention, improved reproducibility
Publications and links to useful resources
CHANGE THE WAY YOU PROCESS CELLS TO GET RELIABLY CONSISTENT DATA.
Assessing immune responses is an indispensable means of evaluating a vaccine’s efficacy during vaccine development. When encountered with a successful vaccine antigen, our immune system is stimulated and produces a complex array of protective mechanisms.
Following a vaccine challenge, changes in frequencies of different immune cell subsets can be determined by flow cytometric immunophenotyping. Functional assays are also used to measure specific immune functions that have been induced by an effective vaccine. The immune status of immune cells can be further evaluated using Intracellular Cytokine Staining, by identifying the specific cytokines and surface markers expressed. In addition to its versatility, flow cytometry enables high flexibility in its applications.
Flow cytometry is a powerful and important technique used in vaccine development, which permits better characterization and analysis of intricate immune responses. It is unique in its ability to measure an unprecedented number of parameters, facilitating simultaneous analysis of multiple immune cell subsets.
Laminar Wash™ technology can be easily incorporated into flow cytometry sample preparation during vaccine development. Automation of cell washing using Laminar Wash™ technology reduces the time needed for sample preparation, as well as increases reproducibility between assays. Gentle cell washing by Laminar Wash technology enhances cell viability and retention, which is especially beneficial when dealing with small starting cell counts or targeting rare immune cell populations. In addition to a higher quality of data, there is an improvement in biosafety as Laminar Wash technology lowers the risk for aerosolization of infectious samples.
“We have used the DA-plate washer to develop a very consistent and time-efficient high throughput thymocyte activation assay.”
Joanna Brzostek, Ph.D., National University of Singapore
Laminar Flow Technology
Comparison of an immuno-staining workflow for the preparation of a single cell suspension for flow cytometry analysis. Laminar Wash technology is equally effective in washing but without the use of a centrifuge.
BENEFITS OF LAMINAR WASH FOR VACCINE DEVELOPMENT
Vaccine research and development have never been more critical for COVID-19 and other emerging diseases. The need for reliable immunological data is critical for vaccine development and clinical trials.
CENTRIFUGE-LESS RED BLOOD CELL LYSIS AND IMMUNOSTAINING WHOLE BLOOD FOR FLOW CYTOMETRY USING LAMINAR WASH™ TECHNOLOGY
DATA USING THE LAMINAR WASH SYSTEMS
Automated Laminar Washing Technology Delivers Good Consistency and Increased Retention of Low K]Input T Cell Numbers from Freshly Thawed PBMC
Improved retention compared to centrifugation. When processing a sample with a lower cell count (10,000 - 100,000 cells), Laminar Washing (datapoint in Red) led to retention of more cells than centrifugation (datapoint in Black). The cell count was compared between each additional cycle of Laminar Washing (9 rounds of 60uL wash buffer exchange) and 1 spin wash cycle.
Retention of More Total Cells and Monocytes with Laminar Wash Technology
Human PBMCs were processed with both Laminar Wash Technology and centrifugation washing methods. Laminar Wash (plate) tends to recover slightly more cells than the centrifugation method with the difference more pronounced with larger monocytes.