Washing Whole Blood Without a Centrifuge – Human and Mouse
Lysing and washing red blood cells using traditional microtiter plates or tubes may result in significant cell loss and damage.
There is now a better way to lyse RBCs while achieving clean antibody staining and lysis as good as or better than traditional methods. The new Laminar Wash™ System offers centrifuge-less blood lysis and leukocyte washing on a single platform, with efficient RBC and debris removal. Whole blood lysis performed by Laminar Wash has similar leukocyte retention compared to lysis with centrifugation, whether enumerated by CD45 staining or FSC-SSC scattering
The scatter plot above is derived from human whole blood lysed and stained on Laminar Wash™ vs. Microtiter plate vs. FACS tube (L to R).
The scatter plot above is derived from mouse blood with TER119 vs. CD45 using Laminar Wash™ vs. Microtiter plate vs. FACS tube (L to R). Note the clean staining using Laminar Wash.
Laminar Wash lets you achieve:
- Consistency by reducing variation from manual handling
- Time savings by completing cell washing in 3 minutes
- Reduced cell stress due to no centrifugation
- Easy integration into your lab automation