Washing Whole Blood Without a Centrifuge – Human and Mouse

Lysing and washing red blood cells using traditional microtiter plates or tubes may result in significant cell loss and damage.

There is now a better way to lyse RBCs while achieving clean antibody staining and lysis as good as or better than traditional methods. The new DA-Cell™ system offers centrifuge-less blood lysis and leukocyte washing on a single platform, with efficient RBC and debris removal. Whole blood lysis performed by DA-Cell has similar leukocyte retention compared to lysis with centrifugation, whether enumerated by CD45 staining or FSC-SSC scattering

The scatter plot above is derived from human whole blood lysed and stained on DA-Cell™ vs. Microtiter plate vs. FACS tube (L to R).

The scatter plot above is derived from mouse blood with TER119 vs. CD45 using DA-Cell™ vs. Microtiter plate vs. FACS tube (L to R). Note the clean staining using DA-Cell.

DA-Cell lets you achieve:

  • Consistency by reducing variation from manual handling
  • Time savings by completing cell washing in 3 minutes
  • Reduced cell stress due to no centrifugation
  • Easy integration into your lab automation