SINGLE-CELL SEQUENCING

Treat your cells gently in preparation for critical single-cell
genomic and proteogenomic applications.

Single Cell Sequencing-new

Laminar Wash technology gently washes cells, minimizing gene expression changes during sample prep.

Improve cell viability and reduce stress on cells; cleaner samples with fewer doublets and debris.

See examples of enhanced cell retention and reduced doublets.

Links to resources and publications.

Overview

CHANGE THE WAY YOU PROCESS CELLS TO
GET RELIABLY CONSISTENT DATA.

Cell clumping, poor retention of cells with already low cell counts, and unwashed residual nucleic acids are just a few symptoms of poor cell washing that impacts single-cell sequencing and proteogenomics. Centrifugation also induces cellular stress on samples, resulting in erroneous gene expression results.

Sample prep is extremely important in single-cell genomics and proteogenomics, especially for single-cell gene expression and immune profiling. While mRNA transcripts are being barcoded, cellular mRNA leakage (increased background) or mRNA degradation (decreased signal) significantly impacts these sensitive measurements. Unlike with flow cytometry, gating out debris or groups of cells is not possible in single-cell sequencing. The cell preparation must be as clean as possible and yield single cells.

Using the Laminar Wash system saves time with fewer manual manipulations. Gently washing cells without unnatural centrifugal forces maintains cell integrity and viability, while ensuring gene or protein expression changes are biological, instead of due to workflow.

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“Curiox (Laminar Wash) really helps retain high numbers of cells”

Dr. Antonino Montalbano, ImmunAI
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Technology

TRADITIONAL CENTRIFUGATION

VS

LAMINAR WASH TECHNOLOGY

Comparison of a cell washing workflow for the preparation of a single-cell suspension for single-cell sequencing analysis. Laminar Wash technology is equally effective in washing but without the use of a centrifuge.

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Benefits

BENEFITS OF LAMINAR WASH FOR SINGLE CELL SEQUENCING & PROTEOGENOMICS

Affordable and simple automation of sample prep by Laminar Wash technology offers many benefits including:

 

  • Enhanced cell retention, especially with low numbers of cells
  • Improved viability and reduced stress on cells
  • Reduced debris and cell clumping (more singlets!)
  • Improved resolution of cell populations
  • Less hands-on time and more high-throughput
Featured Resource

Single-Cell Transcriptomic Analyses Define Distinct Peripheral B Cell Subsets and Discrete Development Pathways. 
Stewart, et al. (2021) Front. Immunol.

Scientific Data

DATA USING THE LAMINAR WASH SYSTEMS

Improved retention, better multiplexing from single cell proteogenomic experiments.
Figure 1: Laminar Wash generates better cell hashing results

Samples processed with a Curiox Laminar Wash system yield fewer doublets, fewer negative results and higher numbers of singlets. Data courtesy of ImmunAI, adapted from Dr. Antonio Montalbano's webinar with Curiox Biosystems.

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Improved Operator Consistency by Automating Only the Wash Step

Figure 2: Laminar Wash Retains Higher Numbers of Cells.

 Laminar Wash consistently retained higher numbers of cells, compared to traditional centrifuge-based methods. This is especially true for samples that have a very low number of cells. Data courtesy of ImmunAI, adapted from Dr. Antonio Montalbano's webinar with Curiox Biosystems..

Recent Publications View All Publications >

    Single-Cell Transcriptomic Analyses Define Distinct Peripheral B Cell Subsets and Discrete Development Pathways

    2021 | Frontiers in Immunology

    The pleiotropic effects of prebiotic galacto-oligosaccharides on the aging gut

    2021 | Microbiome

    CTLA-4 blockade drives loss of Treg stability in glycolysis-low tumours

    2021 | Nature

    Peritoneal Fluid Cytokines Reveal New Insights of Endometriosis Subphenotypes

Recent Publications View All Publications >

    Single-Cell Transcriptomic Analyses Define Distinct Peripheral B Cell Subsets and Discrete Development Pathways

    2020  |  Nature Cancer

    The pleiotropic effects of prebiotic galacto-oligosaccharides on the aging gut

    2020  |  Nature Cancer

    CTLA-4 blockade drives loss of Treg stability in glycolysis-low tumours

    2020  |  Nature Cancer

    Peritoneal Fluid Cytokines Reveal New Insights of Endometriosis Subphenotypes

    2020  |  Nature Cancer

Get Started

AUTOMATE YOUR SINGLE-CELL SEQUENCING WORKFLOW

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FEATURED PRODUCTS

2
Laminar Wash HT2000

A reliable and easy-to-use benchtop instrument for high-throughput flow cytometry labs.

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Laminar Wash AUTO 1000

Complete Automation Solution for Surface Staining and Intracellular Staining for Flow Cytometry.

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Laminar Wash Mini

An affordable, benchtop instrument for low-throughput sample preparation for flow cytometry