One lesson that we’re still learning in our lab is that cells actually can be retained without a centrifuge. Because I was told by multiple people that if you don’t resuspend those cells as part of your wash, it’s not going to work and somehow the cells with the right settings in the Pluto are actually staying retained at the bottom and yet getting washed.

We had automation for antibody cocktailing and data transfer, but washing was always the missing link. We were still carrying plates between disparate instruments, centrifuging, flicking, and resetting the workflow. Pluto finally closed that gap and integrated the full process.

We replaced the standard centrifugation step with Curiox technology. Pluto can allow you to basically simulate centrifuge-free cell washing using a standard liquid handler. It’s really a nice technology.