Flow Cytometry

Get fast, consistent results with higher cell retention, increased resolution, and superior data quality

How Curiox Can Help

  • Automates critical steps in the workflow
  • Cleaner and clearer data
  • Fast, reproducible results
  • Increased cell retention and viability 

Learn more 

Flow cytometry is a powerful analytical tool used to assess physical and chemical properties of cells or particles as they flow through a fluid stream, illuminated by a laser. It plays a vital role in fields such as immunology, hematology, oncology, and molecular biology, supporting both research and clinical applications. 

This technology enables the rapid analysis of individual cells, offering detailed insights into cellular characteristics on a per-cell basis. With the ability to process hundreds to millions of cells per second, flow cytometry is ideal for large-scale studies. It measures multiple parameters simultaneously—such as cell size, granularity, and fluorescence intensity—using fluorescently labeled antibodies or dyes to detect specific cellular markers. Applications include immunophenotyping, cell sorting, cell cycle analysis, apoptosis detection, and disease diagnosis. 

Flow cytometry’s precision, speed, and versatility make it indispensable for laboratories seeking to conduct high-parameter assays and gain deep insights into cellular functions.

Challenges Associated with Flow Cytometry

Despite advancements in laboratory automation, many flow cytometry workflows remain manual, leading to significant pain points. Sample preparation—particularly antibody staining and cell washing—requires multiple steps involving centrifugation, resuspension, and supernatant removal. These labor-intensive processes are prone to variability, affecting consistency and reproducibility, which are critical in high-parameter assays.

The reliance on manual workflows becomes even more challenging as laboratories adopt more complex, multi-parameter panels. In contrast to other automated lab processes, manually managing flow cytometry preparation demands highly skilled technicians, adding pressure amid an industry-wide shortage of experienced personnel. Human errors, such as inconsistent reagent handling or cell loss during centrifugation, further compromise data quality and force costly rework. 

The need to handle sensitive reagents and rare cell populations only increases the difficulty. Manual preparation risks damaging cells or losing them entirely, especially during centrifugation steps, affecting both viability and retention. As automation becomes standard in other lab operations, the continued reliance on manual processes in flow cytometry creates bottlenecks, impeding productivity and data reproducibility.

How Curiox Can Help

Curiox addresses these challenges with its C-FREE and Laminar Wash technologies, which automate critical steps in flow cytometry workflows. These solutions eliminate the need for centrifugation and manual supernatant removal, resulting in fast, reproducible results with enhanced cell retention and viability. By automating labor-intensive processes like cell washing, Curiox reduces the impact of technician variability and ensures consistent, high-quality data. 

Curiox’s platform eliminates manual steps, such as aspiration and flicking, freeing up skilled personnel for more value-added tasks. This automation ensures seamless, reproducible workflows, even for high-parameter assays, where precision is essential. It also minimizes cell loss, preserving rare populations crucial to advanced applications like immuno-oncology and cell therapy. 

With Curiox technologies, labs can keep pace with the increasing demand for high-quality, quantitative data while improving efficiency and productivity. Scientists can focus on research, confident that their automated workflows are delivering consistent, reliable results across every experiment.

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Key Resources

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