C-FREE Technology

C-FREE technology uses laminar flow to delicately wash cells, reducing mechanical stress and preventing cell loss associated with centrifugal methods. This gentle handling is critical for maintaining the physiological states of cells, pivotal for sensitive assays across various applications. The automated process minimizes manual intervention, significantly reducing variability and enhancing consistency across experiments.

Key Benefits
C-FREE Technology

  • Gentle cell handling
  • Enhanced reproducibility
  • Scalability and versatility
  • Regulatory compliance

Learn more

Key Benefits
Pluto System

  • Controlled fluid dynamics
  • Automation ready
  • Enhanced data integrity and reproducibility
  • Eliminates manual processes

Learn more 

Why Change?

The limitations of traditional cell processing methods, like centrifugation, are becoming increasingly apparent. These methods often induce significant mechanical stress and cell loss, which can compromise the integrity and viability of cells—critical factors for the success of sensitive assays. As research demands grow more rigorous, the margin for error narrows. Continuing to rely on outdated techniques not only risks the accuracy of your results, but also hinders the potential for breakthrough discoveries. The time for change is not just necessary; it’s inevitable if you aim to stay at the forefront of scientific advancement.

Why Now?

In today’s fast-paced research environment, the need for precision, reproducibility, and efficiency is greater than ever. As scientific inquiries become more complex, particularly in fields like immunology, cancer research, and pharmaceutical development, the pressure to deliver faster, more accurate results intensifies. Researchers can no longer afford the inconsistencies and manual errors associated with conventional methods. Now is the time to adopt innovative solutions that enhance standardization, reduce variability, and ensure that every experiment is executed with the highest level of precision.

 

The Pluto LT system can significantly streamline our sample preparation workflows. Its automation capabilities could not only improve the accuracy and reproducibility of our flow cytometry data, but also reduce human error during critical steps. As we continue to test across various immune assays, the Pluto system serves to be an essential tool for enhancing efficiency in immune monitoring studies.

Holden Maecker, PhD
Stanford University

Key Benefits of C-FREE Technology

Gentle Cell
Handling

Unlike centrifugation, laminar flow removes supernatants gently, preserving cell integrity and viability.

Enhanced Reproducibility

Automation reduces human error, ensuring that each sample undergoes identical processing.

Scalability and Versatility

C-FREE systems are designed for different throughput needs and can adapt to various laboratory sizes—from research labs to high-throughput clinical settings—without sacrificing sample quality.

Regulatory
Compliance

C-FREE technology is built to meet stringent standards, including 21CFR Part 11*, making it suitable for use in regulated clinical environments and ensuring data integrity.

* Coming soon

Why the Pluto System

The Curiox C-FREE™ Pluto System’s technology (Pluto system) represents a leap forward in cell processing. By harnessing laminar flow to delicately wash cells, C-FREE technology preserves cell integrity and viability, eliminating the mechanical stress and cell loss commonly associated with centrifugal methods. This gentle approach is vital for maintaining the physiological states of cells, which is crucial for the accuracy and reliability of your most sensitive assays.

What sets the Pluto system apart is not just its advanced technology, but its commitment to personalized automation. This feature allows researchers to customize and standardize processes to meet specific experimental needs, dramatically reducing the potential for human error and ensuring consistent, reproducible results across all samples. The automation inherent in the Pluto system enhances error reduction and standardization, offering a level of precision that manual methods simply cannot match.

Moreover, the Pluto system is designed to scale with your needs, whether you’re in a research lab or a high-throughput clinical setting, without sacrificing sample quality. It meets stringent regulatory standards, including 21CFR Part 11* compliance, ensuring that your data is not only accurate but also secure and reliable.

By integrating the Pluto system’s technology into your workflow, you’re not just adopting a new tool—you’re embracing a future where higher cell viability, reduced preparation time, minimized technician hands-on time, and unparalleled reproducibility are the new standard. This is how you achieve deeper insights, faster advancements, and ultimately, how you lead the way in scientific innovation.

* Coming soon

Key Benefits of the C-FREE Pluto System

Controlled Fluid Dynamics

Ensures effective washing while maintaining high particle retention, avoiding the need for pellet formation.

Automation
Ready

Integrates seamlessly with existing liquid handling workstations, turning even basic pipetting robots into sophisticated sample preparation systems.

Enhanced Data Integrity & Reproducibility

Provides consistent results by reducing variability and potential sample disturbance.

Eliminates Manual Processes

Automates the last manual step in lab workflows, freeing up valuable time and enabling high-throughput operations.

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut elit tellus, luctus nec ullamcorper mattis, pulvinar dapibus leo. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut elit tellus, luctus nec ullamcorper mattis, pulvinar dapibus leo.

Name
Organization

Data

Pluto LT system WB sample preparation for flow cytometry analysis. Representative dot plots of one donor WB (technical replicates n=4) processed with conventional centrifuge method or the Pluto LT system are shown. Lower non-CD45+ events in the Pluto LT system-washed samples indicates better wash efficiency compared to the conventional centrifuge method. Population resolution and frequencies are consistent between both methods. Samples were stained with basic immunophenotyping panel (Viability stain eF780, CD45 (HI30) eF506, CD3 (OKT3) SB600, CD4 (OKT4) PerCP-Cy5.5, CD8 (SK1) PE-Dazzle 594, CD19 (HIB19) FITC, CD16+CD56 (B73.1+HCD56) PE, CD11b (ICRF44) BV421, CD11c (Bu15) PE-Cy7) and acquired at a rate of 100 μL/min on an Invitrogen™ Attune™ NxT Flow Cytometer.

Applications

C-FREE technology research areas that are reliant on cellular analysis include:
  • Immunology
  • Cancer research
  • Pharmaceutical development
Benefits that support the needs researchers have for more accurate data, deeper insights and faster advancements are:
  • Ensuring higher cell viability
  • Reducing preparation time
  • Minimizing technician hands-on time
  • Increased reproducibility compared to manual methods

Preserve the quality of your samples with new standards in reproducibility, efficiency, and ease of use with C-FREE technology.

Related Products

  • Pluto System

  • Venus System

Key Resources

Simplify your path to new technology while experiencing customizable automation firsthand

Read More

Breaking a Stereotype: Suspension Cells and Beads May Not Require Centrifugation

Read More

POSTER—2024-A Centrifuge-Less Approach With the Pluto System

Read More

Curiox Biosystems Establishes Innovation Center at Stanford University

Read More

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut elit tellus, luctus nec ullamcorper mattis, pulvinar dapibus leo. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut elit tellus, luctus nec ullamcorper mattis, pulvinar dapibus leo.

Name
Organization