SOFTWARE FOR PLUTO WORKSTATION
Flow cytometry has scaled — more colors, more markers, more sites, more data per sample than ever. Sample prep hasn’t kept up. It’s still done by hand, the same way it was twenty years ago. Workflow Wizard closes that gap. Load your existing staining SOP — no scripting, no re-optimization.
Pluto Workstation runs it automatically. Incubation timing enforced, step sequence locked, every action timestamped. Setup is minutes. Built for scientists, not automation engineers.
Flow cytometry has scaled — more colors, more markers, more sites, more data per sample than ever. Sample prep is the part of the workflow that hasn’t scaled with it. It’s still done by hand, the same way it was twenty years ago. That gap is widening.
In most staining workflows, incubation times are tracked by hand and can drift across operators. Step sequence runs on memory. There is no automated record of what ran, when, or by whom. Most labs don’t routinely run the controls needed to catch this variation, so it goes undetected — until it shows up as unexplained variance downstream that takes days to trace back to technique.
The conventional solution — liquid handlers, robotic arm integrations, centrifuge-dependent systems — carries infrastructure costs and complexity most labs can’t justify. These systems require automation engineers to configure and maintain, not bench scientists. For most labs, the cost of getting there outweighs the benefit.
Workflow Wizard changes that calculation.
WITH WORKFLOW WIZARD
A timestamped run log is generated automatically on the workstation — documenting what ran, in what sequence, and when.
Enter plate type, samples, reagents, and incubation times into a structured input file. The Workflow Generator calculates volumes and builds the run automatically.
Review deck layout, reagent volumes, and plating instructions before committing to a run. This is your verification checkpoint — nothing runs on the instrument until you confirm.
Click Generate & Upload to send a run-ready protocol directly to the Pluto Workstation. One action — protocol transferred, ready to execute.
Step sequence is locked. Incubation timing is enforced by the instrument. Every action is timestamped automatically. Walk away.
Workflow Wizard handles the full range of what a staining protocol actually requires. Protocol definition covers plate type, sample layout, reagents, incubation steps, and buffer preparation — all entered in the structured input file.
Your existing validated SOP is the SOP Workflow Wizard executes — no scripting, no protocol coding, no re-optimization required.
There is no centrifuge required. No robotic arm integration. No automation specialist on staff. Workflow Wizard runs on a compact benchtop workstation — affordable to adopt, and straightforward to operate from day one.
Every run generates a timestamped log automatically on the Pluto Workstation — documenting what ran, in what sequence, and when. Replaces paper records and supports audit-ready documentation.
Plate type, sample layout, reagents, incubation steps, buffer preparation — all defined in the structured input file. Volumes calculated automatically.
Add, remove, or change steps and the plate map updates automatically. No rebuilding from scratch.
Surface staining, intracellular staining, multi-loop protocols, universal and non-universal sample transfers.
C-FREE™ centrifuge-free washing is built in. No external instruments, no sample handoffs, no specialist to operate.
Scales from low to high throughput. Adopt the configuration that fits your lab today.
This matters for scientists who are skeptical of automation. Moving from manual prep to an automated system introduces a legitimate concern — that mechanical error or unfamiliar parameters could compromise data quality. Workflow Wizard addresses this directly.
The Enforcement Mechanism
The Pluto Workstation does not advance to the next step until the protocol-defined incubation time is met. Operators cannot skip steps or change the order of execution. The system enforces the protocol — operator variability is removed by design.
“Standardizing high-parameter flow cytometry workflows has been a persistent challenge in research immune monitoring. The Workflow Wizard addresses this directly: our team can load an existing staining and washing protocol as a table, and the Pluto Workstation executes every step without manual intervention. That combination of immediate usability and run-to-run reproducibility is what makes it a serious tool for high-throughput immune monitoring environments.”
Dr. Holden Maecker
Human Immune Monitoring Center, Stanford University · Scientific Advisor, Curiox Biosystems
Workflow Wizard turns your existing staining SOP into a ready-to-run protocol for the Pluto Workstation — automating cell staining and C-FREE™ centrifuge-free washing without scripting or re-optimization. Pair it with the Antibody Cocktailing Wizard to cover the full sequence — panel definition, cocktail prep, staining, and washing — on a single compact workstation.
Talk to us about how Workflow Wizard fits your existing staining workflow. No configuration required on your end — just bring your protocol.
