TILs

Superior data quality in Tumor-Infiltrating Lymphocytes

How Curiox Can Help

Enhanced yield and data quality
Improved cell separation
Beneficial for low initial cell count samples

Tumor-infiltrating lymphocytes (TILs) are a type of immune cell that has migrated from the bloodstream into a tumor. These lymphocytes, primarily T cells, play a crucial role in the body’s immune response against cancer. The presence, type, and activity of TILs within a tumor can provide important insights into the tumor’s immunogenicity and the patient’s prognosis.

TILs are part of the body’s natural defense mechanism. The most common types include cytotoxic T cells (CD8+), helper T cells (CD4+), regulatory T cells (Tregs), and sometimes B cells and natural killer (NK) cells.

They are clinically relevant as prognostic markers, predictive biomarkers, and therapeutic targets. Isolation and further characterization of TILs are crucial in assessing treatment efficacy in various tumor types. A common challenge arises during downstream processing from the tumor microenvironment (TME). After tissue dissociation and isolation, TILs share their samples with high amounts of debris and dead cells in solution, necessitating stringent washing for contaminant removal.

When isolating TILs from the tumor microenvironment, there are several common concerns, such as:

Cell recovery
Data quality
High background
Aggregation
High levels of debris
Sample prep inconsistency
Workflow inefficiency

How Curiox Can Help

Instead of subjecting these valuable TILs to the high mechanical stress of centrifugation, which can further impact retention, Curiox technologies offer a gentle alternative that can improve yield and data quality, particularly in samples with low initial cell counts.

By automating your cell washing techniques you can ensure reduced debris, cleaner cells, improved separation and superior data quality to help to improve your TILs research results.

Laminar wash gives you a new tool where you are removing more of what you don’t want and keeping more of what you do want in your sample. It’s not magic, but it makes a difference. A difference you can clearly see in your staining results.

Christoph Eberle, PhD, MICR, FRSPH

Charles River Laboratories