HT2100 System

Shorter turnaround time. Washes 96 samples in approximately 4 minutes

Error reduction. Helps to reduce errors from manual pipetting, multiple user touchpoints, and inter-operator variability

Small footprint. Fits into most biosafety cabinets as well as in the AUTO 1000 system

No pelleting of cells. Helps to reduce doublets, clumping, and fluidic system clogging

Improved biosafety. Reduction in aerosolization helps to minimize safety hazards

Data improvements. Reduces debris, yields improved cell segregation, and provides a higher stain index for cleaner resolution of populations

Laminar Wash Technology Enhances Cell Retention and Produces Cleaner, Clearer Data
When dissociating cells from tumor-bearing tissues, tissue debris and enzyme-induced fragments are major factors that can affect the recovery of tumor-infiltrating lymphocytes (TILs). In more invasive and immunologically challenging models, such as mice inoculated with B16F10 melanoma cells, the limited recovery of TILs decreases the likelihood of accurate characterization. The results presented here, using an unchallenged mouse sample and Laminar Wash technology, demonstrate that laminar washing of 1,000,000 tumor-infiltrating cells increases viable cell counts and reduces debris. This cleaner sample preparation method improves lymphocyte recovery to 90.6%, compared to just 10.5% with traditional centrifuge-based washing methods.

Laminar Wash Technology Enhances Neutrophil and Monocyte Retention for Improved Differential Blood Counts
A comparison study using a 16-color flow cytometry panel focused on T cells was conducted with 100 µL of human whole blood using Laminar Wash technology. Whole blood was first lysed with ammonium chloride buffer and then centrifuged once to reduce volume. The samples were aliquoted for antibody incubation and subsequently washed using either the HT2100 system or centrifugation in polystyrene tubes.

Replacing the centrifuge with the HT2100 system in a single wash step provided clear resolution of rare T cell populations, such as MAIT cells, gamma-delta T cells, and regulatory T cells. The study also showed higher retention of neutrophils and monocytes, which was quantified through direct acquisition on a flow cytometer, minimizing cell perturbations.