Antibody Cocktail Prep

Automating cocktail preparation to improve sample preparation processes

How Curiox Can Help

  • Precision and reproducibility
  • Increased productivity
  • Optimal reagent management

Learn more 

Revolutionizing Antibody Cocktail Preparation for Flow Cytometry

Preparing antibody cocktails is a critical step in flow cytometry and other cell analysis workflows, yet it remains time-consuming, error-prone, and highly dependent on manual skills. Curiox offers an innovative solution that fully automates the preparation of antibody cocktails, enabling laboratories to achieve superior consistency, minimize human error, and dramatically improve workflow efficiency.

The Challenges of Manual Antibody Cocktail Preparation

Antibody cocktail preparation typically involves pipetting multiple reagents in precise ratios to create a final staining mix.

Manual preparation is:

  • Labor-intensive and error-prone
  • Limited in scalability, especially for high-parameter panels
  • Prone to variability, which can compromise data quality
 

Additionally, ensuring reproducibility and minimizing reagent waste due to human error—especially for expensive fluorochrome-conjugated antibodies—remains a significant concern for both research and clinical labs.

Curiox’s Automated Antibody Cocktail Solution

Curiox’s C-FREE™ platforms transform the way antibody cocktails are prepared by combining advanced liquid handling with intuitive, CSV-enabled automation. Users can request a fully customized script from Curiox’s scripting team—or, with the optional script generator module, generate scripts themselves by uploading a simple CSV file.

Key Benefits:

  • CSV-Driven Workflows: Upload and execute custom antibody panels via CSV files, allowing rapid setup and maximum flexibility.
  • Precise Dispensing: Achieve exceptional accuracy and reproducibility with low dead volume—ideal for high-value reagents.
  • Scalable Efficiency: Prepare complete antibody cocktails for single panels or high-throughput needs in minutes.
  • Minimized Waste: Engineered to handle small-volume dispensing, reducing reagent consumption without compromising performance.
  • Quality Control Ready: Supports full traceability with automated log files and reports for validation and regulatory needs.
  • Audit Trail & Database Management: Ensures robust data integrity with a built-in audit trail and centralized database for secure tracking of all script executions and changes.
    • High Capacity: Supports up to 40+ antibody reagents in a single prep—ideal for high-dimensional panels and control sets. 
    • Source Vial Compatibility: Compatible with all major commercial antibody vial formats—Transfer to a secondary container not required. 


How the CSV Method Works

With the script generator module, you can quickly produce your own automation scripts using a simple CSV form.

For example:

 Vial Type SourceOutputCurrent Volume (µL)Required Volume (µL)
1BD Glass 5mLSource Rack 1_A1Output Rack 1_A145015.75
2Cytek Tube 5mLSource Rack 1_A2Output Rack 1_A146015.75
3Biolegend TubeSource Rack 1_A3Output Rack 1_A15004.75
4BD TubeSource Rack 1_B1Output Rack 1_A149015.75
5BD Glass 5mLSource Rack 1_C4Output Rack 1_A14805.3
6Biolegend TubeSource Rack 1_B3Output Rack 1_A145029.5
7Biolegend TubeSource Rack 2_A1Output Rack 1_A14307.5
8Biolegend TubeSource Rack 2_A2Output Rack 1_A14207.5
9BD Glass 5mLSource Rack 2_A3Output Rack 1_A14102.44
10BD TubeSource Rack 2_A4Output Rack 1_A14606.4

With the script generator module, you can quickly produce your own automation scripts using a simple CSV form. This CSV tells the system to pipette a specific volume for each antibody as indicated in the table into the same destination well, creating a ready-to-use antibody cocktail in minutes. 

Applications

Our antibody cocktail preparation technology is compatible with a wide range of immunophenotyping and cell analysis workflows, including:

  • High-parameter flow cytometry panel preparation.
  • Spectral flow cytometry.
  • Custom panel prototyping and optimization.


Compatible Systems

The automated antibody cocktail preparation workflow is supported by any Pluto workstation:

  • Pluto HT: High-throughput solution with 96-well capability for maximum panel prep scalability.
  • Pluto MT: Mid-throughput solution with 24- to 72-well capability, ideal for core labs and larger studies.
  • Pluto LT: Low-throughput platform with 8- to 32-well capability, perfect for routine assay development and small-scale projects.

Why Automate Antibody Cocktail Preparation?

Automating the preparation of antibody cocktails:

  • Frees up valuable technician time for higher-value tasks.
  • Improves standardization, reducing variability and human error.
  • Streamlines complex multi-step protocols, enabling faster turnaround.
  • Enhances reproducibility and data quality in every assay. 

Whether you are running small-scale studies or large immunophenotyping projects, Curiox’s automated platforms empower your lab with precision, efficiency, and confidence.

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Key Resources

POSTER—2025 – Clinical Laboratory Automation

POSTER—2025 CYTO – Clinical Laboratory Automation

POSTER—2025 CYTO – Evaluating the Curiox C-FREE Pluto System for Whole Blood Immunophenotyping Sample Processing

Automated Antibody Staining, Now Within Reach

Automated Antibody Staining, Now Within Reach

POSTER—2025 SLAS – Evaluating the Curiox C-FREE Pluto System for Whole Blood Immunophenotyping Sample Processing

POSTER—2025 SLAS – Clinical Laboratory Automation

POSTER—Replacing the centrifuge to increase T-cell recovery and generate consistent data: The Laminar Wash™ approach

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