Antibody Mastermix Prep

Automating master mix preparation to improve sample preparation processes

How Curiox Can Help

  • Precision and reproducibility
  • Increased productivity
  • Optimal reagent management

Learn more 

Revolutionizing Antibody Master Mix Preparation for Flow Cytometry

Preparing antibody master mixes (also known as antibody cocktailing) is a critical step in flow cytometry and other cell analysis workflows, yet it remains time-consuming, error-prone, and highly dependent on manual skills. Curiox offers an innovative solution that fully automates the preparation of antibody master mixes, enabling laboratories to achieve superior consistency, minimize human error, and dramatically improve workflow efficiency.

The Challenges of Manual Master Mix Preparation

Antibody master mix preparation typically involves pipetting multiple reagents in precise ratios to create a final staining mix.

Manual preparation is:

  • Labor-intensive and error-prone
  • Limited in scalability, especially for high-parameter panels
  • Prone to variability, which can compromise data quality


Additionally, ensuring reproducibility and minimizing reagent waste due to human error—especially for expensive fluorochrome-conjugated antibodies—remains a significant concern for both research and clinical labs.

Curiox’s Automated Antibody Master Mix Solution

Curiox’s C-FREE™ platforms transform the way antibody master mixes are prepared by combining advanced liquid handling with intuitive, CSV-enabled automation. Users can request a fully customized script from Curiox’s scripting team—or, with the optional script generator module, generate scripts themselves by uploading a simple CSV file.

Key Benefits:

  • CSV-Driven Workflows: Upload and execute custom antibody panels via CSV files, allowing rapid setup and maximum flexibility.
  • Precise Dispensing: Achieve exceptional accuracy and reproducibility with low dead volume—ideal for high-value reagents.
  • Scalable Efficiency: Prepare complete antibody master mixes for single panels or high-throughput needs in minutes.
  • Minimized Waste: Engineered to handle small-volume dispensing, reducing reagent consumption without compromising performance.
  • Quality Control Ready: Supports full traceability with automated log files and reports for validation and regulatory needs.
  • Audit Trail & Database Management: Ensures robust data integrity with a built-in audit trail and centralized database for secure tracking of all script executions and changes. 


How the CSV Method Works

With the script generator module, you can quickly produce your own automation scripts using a simple CSV form. For example:

Antibody Name

Source Well

Destination Well

Volume (µL)

CD3-BV421

A1

B1

2.0

CD4-PE

A2

B1

2.0

CD8-APC

A3

B1

2.0

This CSV tells the system to pipette 2 µL each of CD3, CD4, and CD8 antibodies into the same destination well (B1), creating a ready-to-use master mix in minutes.

Applications

Our antibody master mix preparation technology is compatible with a wide range of immunophenotyping and cell analysis workflows, including:

  • High-parameter flow cytometry panel preparation.
  • Spectral flow cytometry.
  • Custom panel prototyping and optimization.


Compatible Systems

The automated antibody master mix preparation workflow is supported by any Pluto workstation:

  • Pluto HT: High-throughput solution with 96-well capability for maximum panel prep scalability.
  • Pluto MT: Mid-throughput solution with 24- to 72-well capability, ideal for core labs and larger studies.
  • Pluto LT: Low-throughput platform with 8- to 32-well capability, perfect for routine assay development and small-scale projects.

Why Automate Antibody Master Mix Preparation?

Automating the preparation of antibody master mixes:

  • Frees up valuable technician time for higher-value tasks.
  • Improves standardization, reducing variability and human error.
  • Streamlines complex multi-step protocols, enabling faster turnaround.
  • Enhances reproducibility and data quality in every assay. 


Whether you are running small-scale studies or large immunophenotyping projects, Curiox’s automated platforms empower your lab with precision, efficiency, and confidence.

You May Also Like

  • Pluto ALPHA

  • Pluto Code

  • Pluto Workstation

Key Resources

Automated Antibody Staining, Now Within Reach

Streamline Your Immunophenotyping: IMMUNOLOGY2025™ Poster & Video Access

Automated Antibody Staining, Now Within Reach

POSTER—2025 – Evaluating the Curiox C-FREE Pluto System for Whole Blood Immunophenotyping Sample Processing

POSTER—2025 – Advancing Flow Cytometry Diagnostics

POSTER—Replacing the centrifuge to increase T-cell recovery and generate consistent data: The Laminar Wash™ approach

POSTER—2024 – An Automated, Centrifuge-Less Workflow for Cell Staining and Washing of Flow and Mass Cytometry Samples

POSTER—Semi and fully automated immunostaining sample preparation platforms improve live leukocyte recovery, reproducibility, and flow cytometry data quality

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