WHITEPAPER
Manual pipetting slows you down, wastes costly reagents, and introduces variability that undermines reproducibility.
This whitepaper shows how C-FREE™ Pluto Workstations (LT/MT/HT) automate antibody master mix generation with easy workflow setup and walk-away execution—delivering stain indices equivalent or better than manual prep, lower CVs on key markers, and major reductions in hands-on time—all at costs comparable to conventional pipette workstations.
C-FREE™ Pluto Workstations automate antibody cocktail preparation, staining, and washing all in one station.
See how to save hands-on time, reduce errors, and improve standardization in antibody panel preparation.
How Pluto automates antibody master mix prep with precise dispensing, mixing, aliquoting, and optional FMO/SCC outputs—guided by validated CSV scripts.
Panel definition, duplicate/expiry checks, volume margin checks, and reporting. Learn the required filename rule (CSV = script name) and rapid upload to the tablet.
Compare LT (≤32 wells), MT (≤72), HT (≤96; 4-channel vari-span). Review time savings (~15 min hands-on) and reagent tracking that cuts waste.
In evaluations at Stanford’s Human Immune Monitoring Center, C-FREE Pluto Workstations delivered stain indices equivalent to or exceeding manually prepared 24-color panels, with reduced CV% across key markers including CD3 and CD4. Hands-on time dropped from approximately 90 minutes per batch to around 15 minutes — an 83% reduction — while maintaining full reagent traceability and audit logging. These results held across both internal Curiox studies and independent evaluations at Stanford HIMC, where staff reported consistent staining quality across all major immune subsets in complex multicolor panels.
This whitepaper is relevant for flow cytometry core directors, immunology researchers, and lab managers evaluating automation for antibody panel preparation. It is particularly useful if your lab is:
Running high-dimensional panels (16+ colors) where manual pipetting variability affects reproducibility. Preparing antibody master mixes across multiple operators or sites and seeing batch-to-batch inconsistency. Looking to reduce hands-on preparation time without extensive retraining or new infrastructure. Evaluating whether automated antibody cocktail preparation is compatible with existing SOP requirements and regulatory traceability standards.
The whitepaper covers both the technical workflow and real-world performance data, making it applicable to research labs, core facilities, and CROs running flow cytometry at scale.
Want to see how Pluto could fit into your workflow? Contact our team for a personalized consultation.
