Single cell view of tumor microenvironment gradients in pleural mesothelioma
Giotti, B., Dolasia, K., Zhao, W., Cai, P., Sweeney, R., Merritt, E., Kiner, E., Kim, G. S., Bhagwat, A., Nguyen, T., Hegde, S., Fitzgerald, B. G., Shroff, S., Dawson, T., Garcia-Barros, M., Abdul-Ghafar, J., Chen, R., Gnjatic, S., Soto, A., . . . Tsankov, A. M. (2024). Cancer Discovery, 14(11), 2262–2278. https://doi.org/10.1158/2159-8290.cd-23-0017 Immunotherapies have shown […]
Anti-TIGIT antibody improves PD-L1 blockade through myeloid and Treg cells
Guan, X., Hu, R., Choi, Y., Srivats, S., Nabet, B. Y., Silva, J., McGinnis, L., Hendricks, R., Nutsch, K., Banta, K. L., Duong, E., Dunkle, A., Chang, P. S., Han, C., Mittman, S., Molden, N., Daggumati, P., Connolly, W., Johnson, M., . . . Patil, N. S. (2024). Anti-TIGIT antibody improves PD-L1 blockade through myeloid […]
Immunomodulatory effects and improved outcomes with cisplatin-versus carboplatin-based chemotherapy plus atezolizumab in urothelial cancer
Galsky, M. D., Guan, X., Rishipathak, D., Rapaport, A. S., Shehata, H. M., Banchereau, R., Yuen, K., Varfolomeev, E., Hu, R., Han, C., Li, H., Liang, Y., Vucic, D., Wang, L., Zhu, J., Yu, H., Herbst, R. H., Hajaj, E., Kiner, E., . . . Mariathasan, S. (2024). Cell Reports Medicine, 5(2), 101393. https://doi.org/10.1016/j.xcrm.2024.101393 In metastatic urothelial […]
Disruption of SUV39H1-mediated H3K9 methylation sustains CAR T cell function
Jain, N., Zhao, Z., Koche, R. P., Antelope, C., Gozlan, Y., Montalbano, A., Brocks, D., Lopez, M., Dobrin, A., Shi, Y., Gunset, G., Giavridis, T., & Sadelain, M. (2023). Cancer Discovery, 14(1), 142–157. https://doi.org/10.1158/2159-8290.cd-22-1319 Suboptimal functional persistence limits the efficacy of adoptive T cell therapies. CD28-based chimeric antigen receptors (CARs) impart potent effector function to […]
Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries
Now Watching Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries Elijah W. Chen*1, Chyan Ying Ke*2,3, Joanna Brzostek*1, Nicholas R. J. Gascoigne1, Vasily Rybakin1,41Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 2Singapore Immunology Network, A*STAR, 3Curiox Biosystems, 4Department of Immunobiology, Rega Institute for Medical Research, Katholieke Universiteit (KU) Leuven * These authors […]
Centrifuge-less Whole Blood Staining with Laminar Wash in Biosafety Cabinet
Now Watchinghttps://www.youtube.com/watch?v=bl5M1syUb3M Description Immunophenotyping of whole blood samples using flow cytometry provides valuable pre-clinical and clinical information. Using a centrifuge to process whole blood samples compromises sample quality due to low cell retention and viability. Laminar Wash systems gently retain precious cells without a centrifuge, which is especially true for rare or sensitive immune cell […]
User Manual—HT1000 System
Laminar Wash™ HT1000 Station 96 User Manual
Development of a 43 color panel for the characterization of conventional and unconventional T‐cell subsets, B cells, NK cells, monocytes, dendritic cells, and innate …
Sahir, F., Mateo, J. M., Steinhoff, M., & Siveen, K. S. (2020). Cytometry Part A, 105(5), 404–410. https://doi.org/10.1002/cyto.a.24288 Although many flow cytometers can analyze 30–50 parameters, it is still challenging to develop a 40+ color panel for the phenotyping of immune cells using fluorochrome conjugated antibodies due to limitations in the availability of spectrally unique […]