Applications

Advance Your Sample Preparation with Centrifuge-Free, Automation Solutions

Curiox C-FREE™ technology is designed to make sample preparation more consistent, less labor-intensive, and easier to automate. Whether labs are preparing antibody cocktails, staining cells for flow cytometry, or integrating centrifuge-free washing on liquid handlers, the goal is the same: save hands-on time, reduce manual errors, and make automation practical. 

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Antibody Cocktailing & Sample Preparation

Preparing multi-color antibody cocktails for flow cytometry is a critical step that is often performed by hand. Manual pipetting is time‑consuming and prone to skipped vials or wrong volumes, leading to variability and wasted reagents.

The Pluto Workstation automates antibody master mix preparation, so your panels are generated with robotic precision. The system uses built‑in liquid‑level detection and creates a digital protocol from your antibody list, replacing hours of manual work with a walk‑away workflow. 

Key Benefits:

  • Error‑proof reagent handling – liquid level detection confirms volumes before pipetting to avoid dry aspirates and missed reagents
  • Consistent results – automation eliminates operator variability
  • High-parameter panels – ranging from 1 to 96 antibodies
  • Reagent savings – low dead volume dispensing conserves expensive antibodies
  • Audit trail and traceability – electronic logs provide QA/QC support and regulatory compliance
Antibody Cocktailing
Flow cytometry

Flow Cytometry Sample Preparation

Flow cytometry preparation workflows still rely on manual pipetting and centrifugation. These steps add labor, create bottlenecks, and compromise cell viability. Curiox’s C‑FREE™ Pluto Workstation automates critical steps in the workflow—antibody cocktailing, cell staining, and cell washing.

By replacing centrifugation with precision pipetting, Curiox systems provide full automated sample preparation workflows all in one station. 

Key Benefits:

  • Eliminate centrifugation – cell washing and supernatant removal are performed by controlled wash physics rather than pelleting.
  • All-in-one workstation – full walkaway automation with cocktailing, staining, and washing all in the same liquid handler.
  • Consistent, automated workflows – programmable platforms reduce technician-dependent variability and free up skilled personnel.
  • Scalable throughput – Pluto systems adapt from low-throughput benchtop prep to high-throughput panels.
Flow Cytometry

Centrifuge-free washing on liquid handlers

C FREE™ technology takes the principles of laminar flow and applies them to broader automated liquid handling. Instead of pelleting cells through repeated spin–resuspend cycles, a robotic pipette gently removes supernatant and introduces fresh buffer while cells remain at the bottom of the well. This precise fluid control is ideal for workflows where gentle handling is critical.

Key Benefits:

  • Gentle on cells – Reduce the mechanical stress of centrifugation by replacing it with gentle, laminar-flow washing
  • Standardized automation – Fully automate your workflows to reduce operator variability
  • Seamless integration – Simplify your automation setup by removing complex integrations for centrifugation
  • Proven principles – built on a long-standing foundation of laminar wash principles, C-FREE carries this wash physics to broader automation applications
C-FREE Technology

Automated media exchange with Pluto ALPHA

Refreshing cell culture media often involves manual pipetting and centrifugation—steps that stress suspension or semi-adherent cells and introduce operator variability. The CFREE™ Pluto Alpha system automates media exchange directly within culture plates, using low-shear laminar flow to gently remove and replace media without spin-down.

Key features

  • Gentle automation – smooth laminar flow removes old media and dispenses fresh media without disturbing suspension or semi-adherent cultures (no centrifugation required).
  • Configurable exchange ratios – choose the fraction of media to replace (e.g., 50–88 %) and adjust flow rates for different cell types.
  • Consistent, repeatable results – software-guided robotic movements reduce variability and manual errors.
  • Flexible formats – 24- and 96-well plate compatibility.
  • BSC-friendly footprint – the compact workstation fits inside a biosafety cabinet
Automated Media Exchange

Other applications

Curiox technologies are versatile and support additional applications beyond the featured workflows. Explore other applications below:

Sample types

Our platforms handle a broad range of cell types. Explore sample-‑specific guidance on these pages:

Contact us about your application

Contact us to request product literature, ask technical questions, or set up a call with a specialist to see how C-FREE Pluto Systems can fit into your workflows.