Laminar Wash HT2000 System
Laminar Wash Technology for Improved Sample Preparation for Suspension Cell Assays
Introducing the New Laminar Wash HT2000 System.Built for consistent biosafety and automation.
- Enables biosafety by reducing aerosolization and fits in most biosafety cabinets
- Built for easy integration into automation systems
- Consistency by reducing variation from manual handling
- Time savings by completing cell washing in 3 minutes
- Reduced cell stress due to no centrifugation
- Easy integration into your lab automation
Touch-screen interface to control starting volume, flow rate and number of washes (unlimited).
1 of 4 Optional Buffer Exchanger (BEX) allows user to automatically select buffers from touch-screen interface. No more moving tubes from bottle to bottle. 2 of 4New firmware and PCB allow integration with robotics platforms
3 of 4HT Automation Package
4 of 4
Centrifuge-less whole blood staining with Laminar Wash™ (LW) in Biosafety Cabinet
S LAB Pro with a Curiox Laminar Wash™ (LW) HT2000 System
Cell processing technology enabling superior consistency and robust flow cytometry data using centrifuge-less washing.
The Laminar Wash technology provides an advantage to scientists in processing suspension cells for flow cytometry. Eliminating the usage of the centrifuge has tremendously improved workflows and time management prior to FACs analysis. In the conventional flow cytometry protocol which utilizes the centrifugation method, immune cells undergo numerous washes that generate significant stress to cells. This in turn increases the alteration of biomarker pathways as well as cell loss. With the Laminar Wash technique, cells undergo a gentle wash without added stress, allowing a higher retention of cells for acquisition and cell sorting. In addition, cell surface markers are not modified and unbound antibodies are washed off efficiently, producing cleaner and better segregated data. For high throughput flow cytometry labs, they will be able to achieve consistency by reducing variation from manual handling.
- PBMCs
- Antigen presenting cells (CD40) such as B-cells (CD34, CD45) and dendritic cells
- T-cells – Cytotoxic T cells, Helper T cells (CD3, CD4, CD8)
- Monocytes
- Macrophages
- Cell lines: HEK293T, Yeast cerevisea, CHO, U937, Ramos, Molt4, THP-1, MOM13
- Primary Mouse Tumor Cells
- Mouse Primary Splenocytes
- Colorectal Tumor cells
- Circulating Tumor cells
- Isolated Nucleii
- Thymic Epithelial cells from Mouse
- Mouse Brain cells
- Nucleated red blood cells from maternal blood / cord blood
See How Laminar Wash Technology Works
See Laminar Wash Technology in Action
Here we see a plate dispensed with two drops of green fluorescent polystyrene beads and two drops of food coloring solution. After 20 min of rest, beads all settle to the bottom of wells on the plate. Then a user places the plate in the Laminar Wash HT2000 System and hits the start button. The plate goes in and the fluidics head comes down to a plate. In this run, the case of a washer was lifted intentionally to show the movement of the head and plate inside. The head has 192 nozzles total, 2 nozzles per well, one for dispensing and one for aspiration. During 2 minute duration, it performs 7 times of dispensing and aspiration each. When the wash is complete, a plate comes out. In the pictures, you can see that the food coloring solution completely gone while green fluorescent beads are retained on the plate. You may wonder how come the ink was washed away while beads were retained. Just to clarify, the beads are not attached to a plate. They are naturally and gently sitting on a plate by gravity without any adhesion
Laminar Wash technology enables >95% cell retention and results in superior data over microtiter plates/centrifugation
Direct HTS acquisition by flow cytometer on Laminar Wash 96-well Plates
Equivalent acquisition efficiency and stability as U-bottom plate
Efficient cell mixing even with low cell concentration
Mouse splenocytes (250,000 cells) seeded per well of Laminar Wash plate. Cells were acquired by HTS on BD Celesta flow analyzer, and gated for lymphocytes by FSC-SSC scattering. (Acquisition speed: 1.0uL/s. Mixing volume: 100uL. Mixing speed: 100uL/s. No. of mixes: 5
Washing Whole Blood Without a Centrifuge – Human and Mouse
Lysing and washing red blood cells using traditional microtiter plates or tubes may result in significant cell loss and damage. There is now a better way to lyse RBCs while achieving clean antibody staining and lysis as good as or better than traditional methods. The new Laminar Wash system offers centrifuge-less blood lysis and leukocyte washing on a single platform, with efficient RBC and debris removal. Whole blood lysis performed by Laminar Wash systems has similar leukocyte retention compared to lysis with centrifugation, whether enumerated by CD45 staining or FSC-SSC scattering
The scatter plot above is derived from mouse blood with TER119 vs. CD45 using Laminar Wash HT1000 system vs. Microtiter plate vs. FACS tube (L to R).
Note the clean staining using Laminar Wash System.
Laminar Wash System Specifications
| The Laminar Wash HT2000 System includes the following |
|---|
| Laminar Wash HT2000 Station |
| Integrated Touch Screen 96 Well Fluidic Head |
| Dummy Plate |
| Calibration Plate |
| Filter |
| 0.1m Tubing, Bare |
| 1m Tubing, Red Coded Ring on Connector connected to Filter |
| 1m Tubing, White Coded Ring on Connector |
| Buffer Inlet Bottle Cap |
| 10ml Manual Priming Syringe |
| Allen Key Set |
| Power Adapter (Output 24V, 3A) |
| Electrical Power Cord |
| RS232 to USB Cable |
| User Manual in USB Thumb Drive |
| Declaration of Conformity (for CE compliant) |
| Electronics | |
|---|---|
| Voltage Requirements | 100-200 V |
| Power Consumption | 24V/3.0A |
| Dimensions | |
|---|---|
| HT2000 Dimesions | 513mm H x 262 mm W x 302 mm D 20.2 in H x 10.3 in W x 11.9 in D |
| Weight | 14kg (31 lbs) |