Developing a 43 color panel on Cytek Aurora using Laminar Wash for Sample Prep
The advent of modern flow cytometers and flow cytometric methods has accelerated researchers’ ability to enhance the resolution of samples analyzed. Within a span of 20 years, flow cytometry panels have ballooned from a handful of channels to well over 40. The theoretical limit of flow panels now lies not in the available fluorophores or […]
Development of a 43 color panel for the characterization of conventional and unconventional T‐cell subsets, B cells, NK cells, monocytes, dendritic cells, and innate …
Sahir, F., Mateo, J. M., Steinhoff, M., & Siveen, K. S. (2020). Cytometry Part A, 105(5), 404–410. https://doi.org/10.1002/cyto.a.24288 Although many flow cytometers can analyze 30–50 parameters, it is still challenging to develop a 40+ color panel for the phenotyping of immune cells using fluorochrome conjugated antibodies due to limitations in the availability of spectrally unique […]