Overcoming challenges of Nuclei and Cell Preparation: A scientific perspective by S2 genomics and Curiox Story
Now Watchinghttps://youtu.be/inLymDAn4EI Single-cell transcriptome analysis has been extensively applied in humans and animal models to uncover gene expression heterogeneity between the different cell types of a tissue or an organ. Upstream processing may introduce inherent challenges that mask true analysis and increase user variability. Here we present a simple, yet combinatorial strategy from buffer and […]
Impact of Novel Curiox Laminar Wash on Nuclei Retention and Other Downstream Applications
Now Watchinghttps://youtu.be/U0oWMffKFlM Cell type-specific nuclei populations can be used for a wide range of downstream sequencing applications including transcriptomic and epigenomic analyses. Cerevance’s breakthrough use of Laminar Wash™ Technology in their proprietary NETSseq platform has enabled an improvement in nuclei retention as well as reagent cost reductions and time savings. This Labroots Webinar features David […]
Nuclei Need Nurturing, Too
Laminar Wash™ benefits of automation and gentle cell-based sample preparation can also be extended to nuclei Analytical methods on individual cells provide an enormous amount of useful information. But not all tissue types lend themselves to preparing single cell suspensions necessary for flow cytometry or single-cell ‘omics analysis. Homogenization and dissociation of interconnected tissues such […]