Laminar Wash™ is a core component of the C-FREE™ platform—pioneering centrifuge-less workflows from benchtop to full automation.
For several years now, Laminar Wash™ technology has set the standard for gentle, centrifuge-less cell preparation. Using proprietary laminar flow, it gently washes suspension cells without centrifugation — minimizing mechanical stress, preserving viability, and improving workflow consistency. Validated through extensive research and widespread adoption over the past several years, this technology provides reliable performance with higher cell recovery, improved data quality, and reproducible results across diverse flow cytometry workflows.
Laminar Wash™ is a core component of the C-FREE™ platform — pioneering centrifuge-less workflows from benchtop to full automation. As high-parameter and AI-assisted cytometry workflows demand greater consistency and throughput, labs are graduating from Laminar Wash™ to fully automated C-FREE™ workflows.
Within the C-FREE™ platform, Laminar Wash™ replaces centrifugation with precisely controlled laminar flow — the same fluid control principle that powers all C-FREE™ systems, including Pluto Wash™ automation.
Laminar flow gently removes supernatant while leaving suspension cells undisturbed. Instead of pelleting and resuspending cells, liquid flows smoothly across the plate surface, washing cells in place.
This approach eliminates the mechanical stress, sample loss, and variability introduced by traditional centrifugation — resulting in higher cell recovery, improved viability, and more reproducible data.
By minimizing manual handling, Laminar Wash™ simplifies sample preparation and supports reliable, quantitative results.
Centrifuge-less washing replaces high-force pelleting with controlled laminar flow to gently wash suspension cells. This approach preserves cell integrity, improves recovery, and delivers cleaner, more reproducible data.
By removing centrifugation and manual steps like aspirating and flicking, researchers save up to 70–90% of processing time while reducing variability and human error.
Provides consistently reliable results with higher cell retention and reduces sample variability.
These proven principles now define the C-FREE™ platform—extending gentle, reproducible washing from plate-based Laminar Wash™ systems to fully automated Pluto Wash™ workflows.
The same laminar flow physics established by Laminar Wash™ now forms the foundation of the C-FREE™ Pluto platform, placing both technologies within the same centrifuge-less family built for reproducibility from benchtop to automation.
Laminar Wash™ delivers measurable performance advantages over centrifugation. Comparative data demonstrate improved lymphocyte recovery and clearer population resolution across flow cytometry plots.
Laminar washing delivers better cell retention and cleaner, clearer data — increasing lymphocyte recovery up to 90.6% vs. 10.5% with centrifuge washing.
Representative flow cytometry plots comparing centrifuge-based (top) and Laminar Wash™ (bottom) workflows across lymphocyte, single-cell, live-cell, and marker-gated populations.
Laminar Wash™ advances with neutrophil and monocyte retention for improved blood count.
Representative flow cytometry plots showing enhanced neutrophil and monocyte retention with Curiox Laminar Wash™ Technology (bottom) versus centrifuge-based processing (top).
For several years, Laminar Wash™ has redefined how scientists prepare suspension cells — eliminating centrifugation from workflows while improving data quality and reproducibility.
Today, those same laminar flow principles are carried forward through C-FREE™ technology — powering Pluto Wash™ automation systems for broader, more scalable applications.
While Laminar Wash™ provides reliable, centrifuge-less cell washing in plate-based formats, Pluto Wash™ enables fully automated workflows for applications such as antibody cocktailing, flow cytometry staining, and high-throughput sample preparation.
Learn more about C-FREE™ Technology → | How Laminar Wash™ Powers Pluto →
Compact system for cell washing and media exchange
Full automation workstation for high-throughput cell sample prep
Software integration adding C-FREE™ technology to existing setups
Laminar wash resulted in a two-fold increase in cell retention, at the lower cell inputs, compared to traditional centrifugation protocols. This was important for working with rare and sensitive cell types like T cells, where cell loss can be a significant issue.
Dan Imhoff
Tune Therapeutics
Hundreds of peer-reviewed publications validating Laminar Wash™ and establishing the foundation for C-FREE™ workflows.
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