WHITEPAPER

From Laminar Wash™ to Pluto Wash™ — A Fluid‑Dynamics Validation

Establishing Reliability of C-FREE™ Pluto Wash Technology through Proven Laminar Wash™ Principles: A Fluid Dynamics–Based Validation

Automated. Centrifuge-less. Proven.
This whitepaper demonstrates how the C-FREE Pluto Wash™ platform replicates the trusted Laminar Wash™ physics—delivering equivalent recovery, staining, and reproducibility in a fully automated workflow.

See the data behind centrifuge-less performance for modern flow cytometry sample preparation.

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12-15 min read • Immediate access

At-a-glance findings

  • Overlapping wash‑out curves across Light→Ultra programs, including 5% serum
  • R² ≥ 0.95 concordance for PBMC subset frequencies in Pluto vs Laminar Wash™
  • >90% cell recovery and viability across PBMCs and lysed whole blood
  • Low background in intracellular Ki‑67 with clear positive/negative separation
  • Lower variability and debris carry‑over vs manual centrifugation
  • SBS‑format microplate compatibility for automated liquid handlers

Who benefits from this whitepaper

  • Researchers already familiar with Laminar Wash™ technology who want to understand its extension into the C-FREE™ Pluto Wash platform
  • Multi‑site immunophenotyping programs seeking standardized prep
  • Flow core labs looking to reduce variability and improve reproducibility across users and instruments
  • Translational & regulated labs needing simple, workflow-ready automation

Ready to Take the Next Step?

Want to see how Pluto could fit into your workflow? Contact our team for a personalized consultation.

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