POSTER 2026 – Automated Centrifuge-Free PBMC Washing Flow Cytometry
SCIENTIFIC POSTER Standardizing PBMC Washing in Multi-User Flow Cytometry Labs Using Pluto ALPHA Automated Centrifuge-Free Workflows Manual centrifugation-based PBMC washing introduces operator variability, inconsistent cell recovery, and workflow bottlenecks — especially in multi-user lab settings. This poster presents a head-to-head comparison of conventional centrifugation and automated centrifuge-free washing using the Pluto ALPHA workstation across surface […]
POSTER 2026 – Automated C-FREE Workflow for Reproducible Memory T-cell and NK-cell Immunophenotyping
SCIENTIFIC POSTER Automated C-FREE Workflow for Reproducible Memory T-cell and NK-cell Immunophenotyping Manual staining and wash steps introduce variability in memory T-cell and NK-cell immunophenotyping. This poster evaluates whether automated antibody cocktailing and C-FREE™ sample processing on the Pluto Workstation improve reproducibility — with head-to-head data across stain index, cell retention, and population frequencies.Download the […]
Antibody Cocktailing Automation Cost Model | Technical Note
Automation in Antibody Cocktailing for B-Cell Cytometry Panels See how automation can simplify cocktailing, reduce human errors, and save time. Cole Batty, PhD | Dana-Farber Cancer Institute, Marasco Laboratory Hanwen Melvin Lye, MBA, BCMAS | Senior Director, Scientific Affairs, Curiox Biosystems Manual antibody cocktail preparation becomes increasingly difficult as cytometry panels grow in complexity. Pipetting […]
Validated 384-Well C-FREE™ Washing for High-Throughput Cell Prep
Validated 384-Well Centrifuge-Free Washing for High-Throughput Cell Prep Technical and biological validation of centrifuge-free washing at 384-well scale using the C-FREE™ Pluto platform. Enabling high-throughput cell prep automation Moving to 384-well formats increases throughput and reduces reagent use—but washing at this scale introduces real risks: sample loss, uneven retention, and assay variability. This application note […]
POSTER 2026 – Pluto Code – Enabled Centrifuge-Free Automation: Toward a Universal Standard for Gentle Wash
SCIENTIFIC POSTER This poster presents Pluto Code, a software-defined framework that enables centrifuge-free, gentle cell washing directly on standard liquid-handling platforms. Using C-FREE™ wash physics implemented as executable code, Pluto Code delivers reproducible wash and mix performance across diverse cell-based assays without additional hardware. Validation data demonstrate comparable or improved cell retention, debris removal, and […]
POSTER 2025 – Gentle Media Exchange on an Automated Platform : High Cell Retention, Imaging-Compatible Workflow
Unlock the full poster to see how C-FREE™ Pluto platform transforms media exchange for imaging and flow cytometry workflows. This study demonstrates how gentle, centrifuge-free washing and programmable low-shear media exchange preserve cell integrity, boost retention, and deliver cleaner, more reliable data. Explore side-by-side comparisons with manual methods, insights from whole blood immunophenotyping, and performance […]
POSTER 2025 – Redefining Automated Cell Washing: Centrifuge-Less C-FREE™ Pluto Wash Configurations for Reliable Retention Across Diverse Cell Types
This poster summarizes a side-by-side comparison of manual vs. centrifuge-free workflows for whole blood immunophenotyping. Using automated antibody prep, staining, lysis, and programmable wash steps, the Pluto System achieved comparable cell retention, comparable population frequencies, and improved consistency across donors—all with reduced hands-on time.Download the full poster for detailed methods, figures, and analysis. Fill in […]
Validating C-FREE™ Pluto Wash: Extending Laminar Wash™ Principles
WHITEPAPER From Laminar Wash™ to Pluto Wash™ — A Fluid‑Dynamics Validation Establishing Reliability of C-FREE™ Pluto Wash Technology through Proven Laminar Wash™ Principles: A Fluid Dynamics–Based Validation Automated. Centrifuge-less. Proven.This whitepaper demonstrates how the C-FREE™ Pluto Wash platform replicates the trusted Laminar Wash™ physics—delivering equivalent recovery, staining, and reproducibility in a fully automated workflow. See […]
POSTER – Reducing Operator-Dependent Variability in Immunophenotyping Through Automated Antibody Panel Preparation
Manual antibody cocktailing in high-dimensional flow cytometry often introduces user-to-user variability and consumes valuable hands-on time. This study evaluated an automated workflow using the Curiox C-FREE™ Pluto Workstation for antibody cocktailing, benchmarking it against manual methods across 10- and 24-color panels. Results showed that: Reproducibility improved: Replicate cocktail variation consistently stayed below 5%. Data quality […]
Automating Antibody Cocktail Preparation for Flow Cytometry – White Paper
WHITEPAPER Automating Antibody Cocktail Preparation for Flow Cytometry This whitepaper shows how C-FREE™ Pluto Workstations (LT/MT/HT) automate antibody master mix generation with easy workflow setup and walk-away execution—delivering stain indices equivalent or better than manual prep, lower CVs on key markers, and major reductions in hands-on time—all at costs comparable to conventional pipette workstations. See […]