POSTER 2024 – An Automated, Centrifuge-Less Workflow for Cell Staining and Washing of Flow and Mass Cytometry Samples
Flow cytometry is widely used in research and clinical settings, but automating sample preparation remains a challenge due to the high cost and large footprint of traditional liquid handling systems. Curiox’s Centrifuge-Less (C-FREE™) technology offers a smaller, more cost-effective solution by using laminar flow to wash and stain cells in 96-well plates without centrifugation. C-FREE™ […]
Breathe Easy with Better Models
Now Watching Research for Activating Macrophages and Dendritic Cells with Nanomedicine in Lung Carcinoma https://youtu.be/wljKOIcYsUM Macrophages can mediate phagocytosis of cancer cells and cytotoxic tumor killing, and engage in effective bidirectional interactions with components of the innate and adaptive immune system. Given the continuous recruitment of myelomonocytic cells into tumor tissues, macrophages are candidates for […]
An immunophenotype-coupled transcriptomic atlas of human hematopoietic progenitors
Zhang, X., Song, B., Carlino, M. J., Li, G., Ferchen, K., Chen, M., Thompson, E. N., Kain, B. N., Schnell, D., Thakkar, K., Kouril, M., Jin, K., Hay, S. B., Sen, S., Bernardicius, D., Ma, S., Bennett, S. N., Croteau, J., Salvatori, O., . . . Grimes, H. L. (2024). Nature Immunology, 25(4), 703–715. https://doi.org/10.1038/s41590-024-01782-4 […]
Single cell view of tumor microenvironment gradients in pleural mesothelioma
Giotti, B., Dolasia, K., Zhao, W., Cai, P., Sweeney, R., Merritt, E., Kiner, E., Kim, G. S., Bhagwat, A., Nguyen, T., Hegde, S., Fitzgerald, B. G., Shroff, S., Dawson, T., Garcia-Barros, M., Abdul-Ghafar, J., Chen, R., Gnjatic, S., Soto, A., . . . Tsankov, A. M. (2024). Cancer Discovery, 14(11), 2262–2278. https://doi.org/10.1158/2159-8290.cd-23-0017 Immunotherapies have shown […]
Automated Characterization Method for Validation of Epigenomic Editors in T Cells
Now Watchinghttps://youtu.be/-oRv-U2VMHg To view the transcript click here. Robust characterization methods are important analytical tools for phenotypic characterization in cell and gene therapy. For high-parameter flow cytometry methods, sample preparation is a highly complex and mostly manual procedure. Traditional flow cytometry methods are limited in throughput and resource intensive, which make them suboptimal for discovery screening […]
Disruption of SUV39H1-mediated H3K9 methylation sustains CAR T cell function
Jain, N., Zhao, Z., Koche, R. P., Antelope, C., Gozlan, Y., Montalbano, A., Brocks, D., Lopez, M., Dobrin, A., Shi, Y., Gunset, G., Giavridis, T., & Sadelain, M. (2023). Cancer Discovery, 14(1), 142–157. https://doi.org/10.1158/2159-8290.cd-22-1319 Suboptimal functional persistence limits the efficacy of adoptive T cell therapies. CD28-based chimeric antigen receptors (CARs) impart potent effector function to […]
Nanomedicine-based co-delivery of a calcium channel inhibitor and a small molecule targeting CD47 for lung cancer immunotherapy
Guo, Y., Bao, Q., Hu, P., & Shi, J. (2023). Nature Communications, 14(1). https://doi.org/10.1038/s41467-023-42972-2 Pro-tumoral macrophages in lung tumors present a significant challenge in immunotherapy. Here, we introduce a pH-responsive nanomedicine approach for activating anti-tumoral macrophages and dendritic cells. Using a layered double hydroxide nanosheet carrier, we co-deliver a T-type calcium channel inhibitor (TTA-Q6) and […]
Reinvigoration of Progenitor-Exhausted CD8 T Cells by anti-CTLA-4 Contributes to the Sustained Activity of Combination Checkpoint Blockade
Wang, Kevin and Coutifaris, Paulina and Brocks, David and Solis, Sabrina and Han, Nicholas and Manne, Sasikanth and Kiner, Evgeny and Sachar, Chirag and George, Sangeeth and Yan, Patrick and Kier, Melanie W. and Laughlin, Amy I. and Kothari, Shawn and Giles, Josephine R. and Mathew, Divij and Ghinnagow, Rheem and Alanio, Cecile and Flowers, […]
Future-Proofing Single Cell Applications
Now Watchinghttps://youtu.be/wL2tsRsL9TA Technology has evolved from those early days of batching thousands of cells together to decrypt the millions of base pairs that make up genetic information. Cellular Indexing of Transcriptomes and Epitopes by sequencing (CITE-seq), brings together surface protein phenotyping (similar to flow cytometry) and single-cell RNA sequencing (scRNA-seq). This is achieved by using […]
The Direct Reading Grid – How it Works
Now Watchinghttps://youtu.be/_wdesbdI5ZkIn this video provides walk-throughs on pairing the Direct Reading Grid (DRG) with the Laminar Wash™ (LW) Plate to create the DRG & LW Plate Unit as well as pipetting, handling, transferring, and Big Bear Vortexing with the DRG and LW Plate Unit.